عنوان مقاله [English]
In order to investigating, the interaction effects of cadmium as a heavy metal and salicylic acid as an antioxidant and anti-stress substance in sunflower plant, Sunflower seeds were washed with distilled water after disinfection with 1% sodium hypochlorite solution. Then were planted in pots containing sandy soil in the room, in Environmental conditions with a temperature of 34°C during the day and 25°C at night and 16 hours of light and 8 hours dark photoperiod. This research were performed on a randomized design with four replications under greenhouse conditions.
Sunflower plants in two leaves stage were exposed to Cadmium chloride treatment (0, 50, 100, 150 and 200 µM) each week. One week after cadmium treatment, plant leaves were sprayed with salicylic acid (0, 250 and 500 µM). After harvesting the plants, cadmium concentration in root and shoot was measured. Then antioxidant enzymes were measured. Total protein content of leaf samples was extracted and the pattern of leaf protein changes was investigated by SDS-PAGE electrophoresis. Cadmium concentration in root and shoot increased with increasing cadmium and salicylic acid treatment. Catalase enzyme activity decreased with increasing cadmium concentration, and salicylic acid had a very small effect on its activity. The activity of superoxide dismutase, ascorbate peroxidase and glutathione reductase enzymes increased with increasing cadmium concentration and increasing salicylic acid concentration reduced the activity of these enzymes. Leaf protein electrophoresis under cadmium and salicylic acid treatments showed differences in leaf protein intensity compared to control plants. The expression of some proteins was increased or decreased in the treated plants. Therefore, cadmium and salicylic acid treatments made changes in the amount of cadmium uptake, Antioxidant enzymes activity and the pattern of sunflower leaf proteins.