تأثیر نانو اکسید روی بر مراحل ضدعفونی، استقرار و پرآوری درون شیشه‌ای گیاه بادرنجبویه (Melissa officinalis L.)

نوع مقاله : پژوهشی

نویسندگان

1 گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

2 گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران.

چکیده

کشت بافت گیاهی یکی از مهم­ترین تکنیک­ها در راستای تولید صنعتی متابولیت­های ثانویه می‌باشد. سلول‌های گیاهی یک منبع مناسب و مهم برای تولید انواع متابولیت­های ثانویه با ارزش است. بادرنجبویه (Melissa officinalis L.) یکی از گیاهان دارویی مهم بوده و با داشتن  متابولیت­های ثانویه متنوع در درمان و تسکین ناراحتی­های قلبی، عصبی و گوارشی و بویژه تقویت حافظه و درمان آلزایمر کاربرد دارد. در پژوهش حاضر تاثیر غلظت­های مختلف نانواکسید روی (0، 50، 100، 150 و 200 میلی‌گرم در لیتر) بر مراحل ضدعفونی و زنده­مانی گیاهچه­های بادرنجبویه بررسی گردید. همچنین به منظور بررسی اثر نانوذرات اکسید روی بر مراحل استقرار و پرآوری، گیاهچه‌های بادرنجبویه در محیط­های کشت موراشیک اسکوگ­ حاوی نانوذرات اکسید روی در چهار غلظت­ مختلف (صفر، 25، 50 و 75 میلی­گرم در لیتر) کشت شدند. بر اساس نتایج بدست آمده از این پژوهش کاربرد 200 میلی­گرم در لیتر باعث کاهش معنی­دار آلودگی­های باکتریایی و قارچی شد و تعداد گیاهچه­های سالم در مقایسه با سایر تیمار­ها بیشتر بود. همچنین بر اساس نتایج آزمایش دوم، با افزایش غلظت نانو ذرات اکسید روی میزان رشد و پرآوری کاهش پیدا کرد. از میان غلظت­های مختلف نانوذرات، غلظت­های 25 میلی­گرم بر لیتر نانو اکسید روی بیشترین تأثیر در افزایش معنی­دار میزان کلروفیل و غلظت­های 25 و50 میلی­گرم بر لیتر بیشترین تاثیر را در افزایش کاروتنوئیدها داشت. طبق نتایج بدست آمده در این تحقیق کاربرد نانوذرات اکسید روی با کمترین غلظت (25 میلی­گرم بر لیتر)­، باعث افزایش جذب آب و املاح معدنی شده و در نهایت منجر به افزایش رشد و پرآوری گیاهچه­های بادرنجبویه گردید.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of zinc oxide nanoparticles on sterilization, establishment, and proliferation of lemon balm (Melissa officinalis L.)

نویسندگان [English]

  • Ehsan Soraya 1
  • Gholamreza Gohari 2
  • Alireza Motallebi Azar 1
  • Saeedeh Alizadeh Saleteh 1
1 Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Department of Horticulture, Faculty Agriculture, University of Maragheh, Maragheh, Iran.
چکیده [English]

Plant tissue culture is one of the most important techniques for the production of secondary metabolites. Plant cells are an important and appropriate source for the production of various valuable secondary metabolites. Melissa officinalis L. is an important medicinal plant with applications in treatment and alleviation of heart, nervous system, and gastrointestinal diseases, and particularly in memory enhancement and Alzheimer. This study investigated the effect of various concentrations of zinc oxide nanoparticles (0, 50, 100, 150, and 200 mg l-1) on disinfection stages, establishment, and proliferation of the lemon balm. Also, in order to study the effects of zinc oxide nanoparticles on establishment and proliferation of the lemon balm, explants were cultivated in murashige and skoog media containing zinc oxide nanoparticles at four different concentrations (0, 25, 50 and 75 mg L-1). Results showed that application of 200 mg l-1 zinc oxide nanoparticles significantly reduced fungal and bacterial infections and the number of healthy plantlets was more compared to the other treatments. Also, the second experiment showed that with an increase in the concentration of zinc oxide nanoparticles, the growth and proliferation decreased. Among different concentrations of zinc oxide nanoparticles, 25 mg L-1 had the maximum effect with significant increase in chlorophyll content while 25 and 50 mg L-1 zinc oxide nanoparticle concentrations had the maximum effect on increasing carotenoid contents. According to the findings,خطای ترجمه application of zinc oxide nanoparticles at low concentration (25 mg L-1) improved water and mineral uptake and eventually resulted in an improved growth and proliferation of Melissa officinalis L. plants

کلیدواژه‌ها [English]

  • Nanoparticle
  • Contamination
  • Chlorophyll
  • Lemon Balm
  • Zinc
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