بررسی اثر محیط غرقاب و خشک بر ظهور نواحی ژنی کنترل کننده صفات گیاهچه‌‌های برنج (Oryza sativa L.) با استفاده از نشانگرهای SSR

نوع مقاله : پژوهشی

نویسندگان

1 گروه تولیدات گیاهی، دانشکده کشاورزی، دانشگاه گنبد کاووس، گنبد کاووس، ایران

2 گروه کامپیوتر، دانشکده علوم پایه و فنی ‌مهندسی دانشگاه گنبد کاووس، گنبد کاووس، ایران

چکیده

شناسایی ژن‌های مرتبط با تحمل به تنش‌ها و مکانیسم‌های تحمل به آن‌ها یک عامل مهم برای ایجاد مقاومت در گیاهان است. به‌همین منظور؛ آزمایشی با استفاده از 99 ژنوتیپ برنج در سه تکرار در قالب طرح کاملاً تصادفی در شرایط غرقاب و تنش خشکی در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه گنبد کاووس در سال 1397 اجرا شد. در شرایط غرقاب در طول دوره رشد گیاه آبیاری انجام شد. برای اعمال تنش خشکی، آبیاری از مرحله سه برگی قطع شد. با توجه به منحنی رطوبت، میزان تنش 2 درصد رطوبت وزنی معادل 55/0- مگا پاسکل تخمین زده شد. صفات ارزیابی­شده در هر دو شرایط کشت تفاوت معنی‌داری با هم داشتند. تجزیه ارتباط اطلاعات به‌دست آمده از آزمایش‌های مولکولی و بررسی‌های فنوتیپی در شرایط غرقاب نشان‌دهنده این موضوع بود که از میان آلل‌ها، آلل RM129A با وزن تر ساقه‌ و طول ریشه‌ و آلل RM129G با وزن‌تر ریشه‌ و عرض بزرگ‌ترین برگ و آلل RM129B با وزن خشک ریشه‌ و طول بزرگ‌ترین برگ و آلل RM1029I با وزن خشک ساقه‌ و طول بزرگ‌ترین برگ و در شرایط تنش خشکی آلل RM1029G با وزن خشک ساقه‌، حجم ریشه‌ و تعداد ریشه‌ دارای بیش‌ترین ارتباط با صفت‌های مورد ارزیابی در آزمایش بودند. پرایمر RM1029 یکی از پرایمر‌های مهم در مرحله گیاهچه شناسایی شد. از نتایج حاصل از این پژوهش می‌توان در جهت افزایش تحمل به خشکی ارقام برنج استفاده نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the effects of flooding and drought environment on the emergence of gene regions controlling rice (Oryza sativa L.) seedling traits using SSR markers

نویسندگان [English]

  • bahareh ghasemi 1
  • hossein sabouri 1
  • hossein hossein moghaddam 1
  • Abbas Biabani 1
  • mohamad javad Shykh zadeh 2
1 Department of Plant Production, College of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad Kavous, Iran
2 Department of Computer, College of Basic Sciences and Technology Engineering, Gonbad Kavous University, Gonbad Kavous, Iran
چکیده [English]

Identification of genes related to stresses tolerance and mechanisms of tolerance is an important factor for developing tolerant plants. For this purpose, an experiment was conducted based on completely random design with three replications with 99 rice genotypes under flooding and drought stress at Gonbad Kavous University in 2018. To apply drought stress, irrigation was stopped at the three-leaf stage. According to the humidity curve, the stress level of 2% by weight moisture was estimated to be -0.55 MPa. Significant differences were detected between traits in both conditions. The result of association analysis of molecular and phenotype studies under flooding condition revealed that among the alleles, the RM129A allele with stem fresh weight and root length, the RM129G allele with root fresh weight and the width of the largest leaf, the RM129B allele with root dry weight and the length of the largest leaf, the RM1029I allele with stem dry weight and the length of the largest leaf, and under the drought condition, the RM1029G allele with stem dry weight, root volume, and the number of roots were maximally associated with the relevant traits under study. RM1029 was recognized as one of the most important primers at the seedling stage. The results of this research can be used to increase drought tolerance in rice varieties.
 

کلیدواژه‌ها [English]

  • Association analysis
  • Drought stress
  • Genetic variation
  • Rice
  • seedling

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فیزیولوژی محیطی گیاهی
دوره 16، شماره 61
خرداد 1400
صفحه 1-16

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