تغییرات شاخص‌های جوانه‌زنی و فعالیت سیستم آنتی‌اکسیدانتی بذرهای پنبه (Gossypium hirsutum L.) طی زوال

نوع مقاله : پژوهشی

نویسندگان

1 گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 گروه زیست‌شناسی، دانشگاه گلستان، گرگان، ایران

چکیده

بذرها در طی انبارداری زوال یافته و پیر می­شوند به طوری که سرعت  این فرآیند به دما و رطوبت بذر در طی انبارداری بستگی دارد. این آزمایش به منظور مطالعه فعالیت سیستم آنتی اکسیدانتی در بذرهای پنبه رقم ارمغان در طی زوال صورت گرفت. برای ایجاد سطوح مختلف زوال از آزمون تسریع پیری استفاده شد. بذرهای پنبه به‌مدت 0، 48، 96، 144 و192 ساعت در دمای 43 درجه سانتی­گراد و رطوبت نسبی 100 درصد قرار گرفتند. نتایج نشان داد با افزایش دوره زوال هدایت الکتریکی غشاء، پراکسیداسیون لیپید و پراکسید هیدروژن افزایش یافت. افزایش پراکسید هیدروژن همراه با کاهش فعالیت آنزیم کاتالاز و افزایش فعالیت آنزیم پراکسیداز و مقدار اسید آسکوربیک بود که نشاندهنده تاثیر کاهنده دوره زوال بر فعالیت آنزیم کاتالاز نسبت به آنزیم پراکسیداز و اسید آسکوربیک بود. همچنین با افزایش دوره زوال درصد، سرعت و یکنواختی جوانه­زنی کاهش یافت. به­طور کلی نتایج این تحقیق بیانگر این مطلب است که یکی از دلایل اصلی زوال بذر در بذرهای پنبه، تنش اکسیداتیو ناشی از تجمع گونه­های اکسیژن فعال و کاهش کارایی سیستم آنتی اکسیدانتی می ­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Changes in germination indices and the activity of antioxidant system of cottonseed (Gossypium hirsutum L.) during deterioration

نویسندگان [English]

  • Farshid Ghaderifar 1
  • omid sancholi 1
  • Hamidreza sadeghipour 2
1 Department of Agronomy, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 Department of Biology, Golestan University, Gorgan, Iran
چکیده [English]

Seeds deteriorate and become aged during storage so that the rate of this process depends on the temperature and seed moisture content. This experiment was conducted to study changes in the activity of antioxidant system of cottonseeds during deterioration. An Accelerated aging test was used to create different levels of deterioration. Cottonseeds were incubated at 43 ℃ for 0, 48, 96, 144, and 192 hours and 100% relative humidity. Results showed that the membrane electrical conductivity, lipid peroxidation, and hydrogen peroxide increased with lengthening of deterioration periods. Increase in hydrogen peroxide was accompanied with decreased activity of catalase and the increased activity and content of peroxidase and ascorbic acid, respectively, which indicates declined activity of catalase due to aging as compared with peroxidase activity and ascorbic acid content. Also, with an increase in the period of deterioration, percent, rate, and uniformity of germination reduced. In general, the study indicated that the oxidative stress due to the accumulation of reactive oxygen species and the reduction of the antioxidant system is one of the main reasons for cottonseed viability loss during storage.

کلیدواژه‌ها [English]

  • Aging
  • Oxidative stress
  • reactive oxygen species
  • seed storage
  • seed vigor
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