ارزیابی تحمل به تنش یخ زدگی گیاه پوششی فیلا (Phyla lanceolata) تحت تاثیر برخی مالچ‌های آلی در شرایط کنترل شده

نوع مقاله : پژوهشی

نویسندگان

1 گروه باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

به منظور بررسی اثر تنش یخ زدگی در گیاه پوششی فیلا، تحت تاثیر برخی مالچ‌های آلی، این آزمایش به صورت فاکتوریل بر پایه طرح کاملاً تصادفی با چهار تکرار در دانشکده ی کشاورزی دانشگاه فردوسی مشهد اجرا گردید. پنج سطح دمای یخ زدگی (0، 5-، 10-، 15- و 20- درجه سانتیگراد) و سه نوع مالچ (دامی، سوزنی برگ و سبوس) تیمارهای این آزمایش بودند. جهت ارزیابی تحمل به دماهای یخ زدگی صفاتی چون درصد نشت الکترولیت‌ها، مقدار کربوهیدرات محلول و پرولین برگی، تعداد برگ، سطح برگ و وزن خشک بوته در نظر گرفته شد. نتایج نشان داد اثرات ساده و متقابل مالچ و دما صفات مورفولوژیکی اندازه گیری شده، میزان نشت الکترولیت، محتوای نسبی آب برگ گیاه فیلا، عدد اسپد و مقدار رنگدانه‌های فتوسنتزی (کلروفیل a، b و کل) معنی دار شد. تمام مالچ‌های آلی به کار رفته در این پژوهش منجر به افزایش وزن خشک بخش هوایی و ریشه گیاه فیلا در دوره رشد پس از تنش یخبندان شد. به‌طوری‌که با کاربرد سه مالچ سبوس، سوزنی برگ و دامی وزن خشک هوایی از 15/2 گرم در شرایط عدم استفاده از مالچ به 72/2، 13/3 و 19/3 گرم در هر بوته و وزن خشک ریشه از 50/1 گرم به 90/1، 23/2 و 19/2 گرم در هر بوته رسید. بیشترین نشت الکترولیت سلول‌های برگی (80%) در دمای 20- درجه و گیاهان تحت تیمار مالچ سبوس و بیشترین محتوای نسبی آب برگ (55%) در دمای 0 درجه و گیاهان تحت تیمار مالچ سوزنی برگ بود. همچنین مقدار کلروفیل کل در شرایط استفاده از مالچ کود دامی 11/0 میلی گرم بیشتر از شاهد بود. به طور کلی در بین مالچ‌های استفاده شده مالچ کود دامی تاثیر بیشتری بر بهبود اثرات ناشی از یخبندان بر صفات مورفولوژیکی و بیوشیمیایی گیاه داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the effect of freezing stress tolerance of Phyla lanceolata under some organic mulch in controlled conditions

نویسندگان [English]

  • maryam kamali 1
  • Yahya Selahvarzi 1
  • Jafar Nabati 2
1 Horticulture department, Ferdowsi University of Mashhad, Mashhad, Iran
2 Institute of Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

In order to investigate the effect of frost stress on Phyla lanceolata as a cover plant under the influence of some organic mulches, this experiment was conducted as a factorial experiment based on completely randomized design with four replications at Ferdowsi University of Mashhad. Five levels of freezing temperature (0, -5, -10, -15, and -20 °C) and three types of mulch (manure, leaf needle, and bran) were treated in the experiment. For evaluation of frost tolerance temperatures, some traits such as electrolyte leakage percentage, soluble carbohydrate content, leaf proline content, leaf number, leaf area, and plant dry weight were considered. Results showed that the simple and interaction effects of mulch and temperature on the measured morphological traits, electrolyte leakage, relative water content, spade and photosynthetic pigment content (chlorophyll a, b and total) were significant. All of the organic mulches used in the study increased shoot and root dry weight so that by applying three mulch of bran, leaf needle, and animal manure, shoot dry weight from 2.15 g under non-mulch conditions reached to 2.72, 3.13, and 3.19 g/plant, respectively and root dry weight from 1.50 g reached 1.90, 2.23, and 2.29 g /plant, respectively. The highest electrolyte leakage (80%) was at -20 °C and in plants treated with bran mulch and the highest relative water content (55%) was at 0 °C and in plants treated with needle mulch treatment. Also, total chlorophyll content was 0.11 mg higher than that in control treatment. In general, among the mulches used, animal manure mulch had the greatest effect on mitigating the effects of frost on morphological and biochemical traits of the plant.

کلیدواژه‌ها [English]

  • Carbohydrate
  • Chlorophyll
  • dry weight
  • Electrolyte leakage
  • Phyla lanceolata
  • Proline
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