بررسی اثر پیش تیماری توام کلرور سدیم و زمان بر رشد، وضعیت رنگیزه‌های فتوسنتزی و نسبت‌های فتوسیستمی در سیانوباکتریوم خاکزی Calothrix sp. FS 65

نوع مقاله: پژوهشی

نویسندگان

1 گروه زیست شناسی ، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

2 گروه شیمی ، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

چکیده

در این تحقیق امکان تغییر در رشد، توده زنده و رنگیزه­های فتوسنتزی سیانوباکتری خاکزی Calothrix sp. FS 65، از طریق دو عامل پیش تیمار با غلظت‌های مختلف (17، 80 و 160 میلی‌مولار) کلرور سدیم و زمان (24 و 96 ساعت) بررسی شد. سیانوباکتری پس از تخلیص به مدت 24 ساعت و 96 ساعت در شوری 17، 80 و 160 میلی مولار کلرور سدیم قرار گرفت و سپس به محیط کشت عادی فاقد نمک اضافی منتقل گردید. سنجش‌ها شامل بقا و رشد، سنجش‌های رنگیزه‌ای در زیوه (فیکوسیانین، آلوفیکوسیانین، فیکواریترین، کلروفیل) و مقایسه تاثیر توام زمان و شوری بر نسبت‌های فتوسیستمی نمونه بود. نتایج نشان داد که پیش تیمارهای 24 ساعت، قادر به حذف کامل فاز تاخیری در رشد نمونه بودند. با توجه به نقش فاز تاخیری در خوگیری از طریق ایجاد ساختارهای لازم فعالیت نیتروژنازی و نیز پوشش‌های هوشمند برون سلولی، حذف فاز تاخیری می­تواند دستاورد قابل توجهی تلقی گردد. محتوای اجزای سازنده فیکوبیلی­زوم یعنی فیکوسیانین، فیکواریترین و آلوفیکوسیانین تحت تاثیر 96 ساعت در شوری 160 میلی مولار تا بیش از 60 درصد افزایش یافت. نسبت فتوسیستم یک به دو در شوری 17 میلی­مولار و زمان 24 ساعت به بیشینه خود رسید و در شوری 160 میلی‌مولار و 96 ساعت کاهش معنی­دار داشت. در زمان‌ها و تیمارهای بینابینی اختلاف وجود داشت ولی معنی‌دار نبود. روی هم رفته استفاده از پیش تیمارهای ساده شوری و زمان توانست کارایی انتقال انرژی در فتوسیستم‌ها و  تولید انرژی و ردکتان را  افزایش دهد که برای بیوتکنولوژی کشت‌های انبوه امتیاز بزرگی محسوب می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Studying of the Pretreatment Effects of the combination of Sodium Chloride and Time on Growth, Photosynthetic Pigmentation Status and Photosystems Ratios in Cyanobacterium Calothrix sp. FS 65

نویسندگان [English]

  • Hamideh Sadat Amirlatifi 1
  • Shadman Shokravi 1
  • Aryan Sateei 1
  • Mazyar Ahmadi Golsefidi 2
  • Mehrali Mahmoudjanlo 1
1 Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
2 Department of Chemistry, Gorgan Branch, Islamic Azad University, Gorgan, Iran
چکیده [English]

Possibility of the growth, biomass and photosynthetic pigment content fluctuations were surveyed in the soil cyanobacterium Calothrix sp. FS 65 via the combinations of two parameters means different concentration of salinity (17 , 80 and 160 mM) and time (24 and 96 hours). After purification, axenic culture of the cyanobacterium was pretreated with 24 and 96 hours in 17, 80, and 160 mM NaCl and then moved to usual culture media without extra salinity. Analysis included survivality and growth, in vivo pigment (phycocyanin, allophycocyanin, phycoerythrin and chlorophylls) and comparison of the combination of time and salinity on photosystem ratios. Results showed that the 24 hours pretreatments cause the complete deletion of the lag phase of the growth curve of such a strain. This may be an outstanding result regarding the role of lag phase on the acclimation processes and essential compartments of the nitrogenase and meanwhile intelligent outer layers envelopes. The main components of phycobilisomes means phycocyanin, phycoerythrin and allophycocyanin increase more than sixty percent after 96 hours pretreatment in 160 mM salinity. The phosystems ratio reached to the maximum at 24 hours after 17 mM salinity and then decreased significantly at the 96 mM at 96 hours after inoculation. The difference between the intercalary times and treatments seems insignificance despite that there was obvious difference. Collectively, using pretreatments may have considerable effects on the energy transfer in photosystems and the production of the energy and reductans which seem the great advantageous for the large scale cultivation technology.

کلیدواژه‌ها [English]

  • Calothrix
  • Cyanobacteria
  • Eco physiology
  • Pretreatment
  • Salinity
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