اثر بازدارندگی تنظیم کننده‌های رشد مختلف روی بیماری کپک آبی در ارقام سیب (Malus × domestica Borkh.)

نوع مقاله: پژوهشی

نویسندگان

1 پژوهشکده میوه‌های معتدله و سردسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران

چکیده

میوه سیب (Malus × domestica Borkh.) هم به صورت مصرف تازه و هم در تولید محصولات فرآوری شده کاربرد فراوانی دارد. عموماً محصول برداشت شده پس از یک تا چند ماه انبارداری وارد بازار می‌شود که این باعث اهمیت محافظت محصول در مقابل بیماری‌های پس از برداشت (به خصوص در کشاورزی ارگانیک) شده است. یکی از این بیماری‌ها کپک آبی (Penicillium expansum) است که به دلیل گسترش بالا و تولید ماده سرطانزای پاتولین از اهمیت شایان توجه‌ای برخوردار است. در این تحقیق تاثیر اسپری پاشی تنظیم کننده‌های رشد اسید آبسزیک، اسید سالیسیلیک، متیل جاسمونات، بتا آمینوبوتیریک اسید و اسید کربوکسیلیک روی این بیماری در ۱۰ رقم سیب مورد بررسی قرار گرفت. آلودگی مصنوعی روی میوه‌ها با استفاده از سوسپانسیون قارچ ایجاد و سپس نمونه‌ها با تنظیم کننده های رشد با غلظت های متفاوت اسپری شدند. صفات فنولوژیکی مانند سفتی میوه و درجه نرم‌شدگی میوه‌ها در حین انبارداری و سطح آلودگی اندازه‌گیری شدند. داده‌های پژوهش به صورت آزمایش فاکتوریل با دو عامل رقم و تنظیم کننده رشد در قالب طرح کاملا تصادفی با بسته نرم افزاری R مورد تجزیه و تحلیل قرار گرفتند. نتایج حاکی از وجود تفاوت معنی‌دار بین ارقام برای فاکتورهای مورد بررسی بود. علاوه‌ بر متیل جاسمونات، اسید سالیسیلیک و بتاآمینوبوتیریک اسید بیشترین اثر بازدارندگی را روی این بیماری نشان دادند. تاثیر دو تنظیم کننده رشد اسید آبسیزیک و کربوکسیلیک ‌اسید نیز بسته به رقم متفاوت بود و در بعضی از تیمارها هیچ گونه تاثیر معنی‌داری نشان ندادند. نتایج این تحقیق می‌تواند در مطالعات به نژادی برای شناخت بهتر مکانسیم مقاومت در این گیاه مورد استفاده قرار گیرد و همچنین به درک بهتری از ارتباط این مواد با پاسخ‌های فیزیولوژیک و در نهایت ایجاد مقاومت به بیماری در سیب کمک نماید.
 

کلیدواژه‌ها


عنوان مقاله [English]

Inhibitory effect of different plant growth regulators on blue mold disease in apple (Malus × domestica Borkh.)

نویسندگان [English]

  • Mansureh Keshavarzi 1
  • Saeid Mirzaei 2
  • Masoud Ahmadi-Afzadi 2
1 Cold and Temperate Fruits Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization, Karaj, IranIran
2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
چکیده [English]

Apple (Malus × domestica Borkh.) is one of the most economically important crops which is widely used either as fresh fruit or as processed foods. Apple fruit is usually introduced to the markets after a storage period of several months. This makes it important to protect fruits against the post-harvest diseases specially in organic production. Blue mold (Penicillium expansum) is one of these diseases, which due to spreading very fast in storage and producing a carcinogenic compound, called patulin, is of particular importance. In the present study, the impact of methyl jasmonate, abscisic acid, salicylic acid, beta-aminobutyric acid, and carbocyclic acid sprayed was investigated on blue mold. Apple fruits of 10 cultivars were surface sterilized and inoculated with spores of fungus, and then were treated with the plant growth regulators. The fruit firmness, softening rate and lesion decay were evaluated. Data were analyzed in a factorial design with two factors i.e., cultivar and plant growth regulator in a completely randomized design in R software package. The impact of hormones was significant on controlling the blue mold, and three plant growth regulators, methyl jasmonate, salicylic acid, and beta-amino butyric acid had the greatest inhibitory impact on the disease. Carboxylic acid and abscisic acid had contradictory impacts on different cultivars, and did not result in significant impacts in some cultivars. The results of this study can be used in the breeding programs in order to help better understand plant disease mechanisms. They can also be applied as a practical method to control blue mold in apple.

کلیدواژه‌ها [English]

  • apple
  • blue mold
  • physiology
  • Plant growth regulator
  • Resistance

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