ارزیابی فعالیت آنتی‌اکسیدانی و خصوصیات بیوشیمیایی گیاه دارویی شنبلیله (Trigonella foenum- graecum L.) در واکنش به سولفات آهن و بیوپتاس تحت تنش خشکی

نوع مقاله: پژوهشی

نویسندگان

1 گروه آموزشی علوم باغبانی و مرکز پژوهشی گیاهان ویژه منطقه دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

2 گروه علوم باغبانی، گرایش فیزیولوژی گیاهان دارویی، ادویه‏ای و عطری، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران

چکیده

به‌منظور ارزیابی فعالیت آنتی‌اکسیدانی و خصوصیات بیوشیمیایی شنبلیله در واکنش به سولفات آهن و بیوپتاس تحت شرایط تنش خشکی، آزمایشی، به صورت صورت کرت­های خرد شده در قالب فاکتوریل بر پایه بلوک­های کامل تصادفی انجام شد. تیمارها شامل: تنش خشکی (شامل آبیاری نرمال به عنوان عدم تنش و قطع آبیاری در اوایل ­گلدهی و میوه‌دهی به‌مدت 14 روز به­عنوان تنش خشکی) به‌عنوان عامل اصلی و تیمار‌های کودی بیوپتاس (0 و 5 کیلوگرم در هکتار) و سولفات آهن (0 و 5/1 در هزار) به عنوان عوامل فرعی در نظر گرفته شدند. نتایج نشان داد که تنش خشکی، بیوپتاس و سولفات آهن و برهمکنش آنها بر میزان صفات آنتوسیانین، ترکیبات فنولی و فلاونوئیدی معنی‌دار گردید، بطوریکه بالاترین میزان آنتوسیانین شنبلیله از تیمار‌های 5 کیلوگرم بیوپتاس و 5/1 در هزار سولفات آهن در شرایط تنش خشکی و کمترین میزان آنها در شاهد و تحت شرایط تنش بدست آمد. همچنین در شرایط تنش خشکی، ترکیبات فنولی و فلاونوئیدی شنبلیله افزایش یافت، بطوری‌که بیشترین میزان ترکیبات فلاونوییدی شنبلیله در شرایط تنش خشکی و از تیمار‌های 5 کیلوگرم بیوپتاس و 5/1 در هزار سولفات آهن حاصل گردید. اثر متقابل تنش خشکی و سولفات آهن هم بر فعالیت آنتی‌اکسیدانی گیاه معنی‌دار شد، بطوری‌که بیشترین میزان فعالیت آنتی‌اکسیدانی با میزان 8/62 درصد در شرایط تنش خشکی و تیمار سولفات آهن 5/1درهزار مشاهده گردید. همچنین بیشترین مقدار قندهای محلول شنبلیله در تنش خشکی و در تیمار 5 کیلوگرم بیوپتاس و کمترین مقدار این صفت در شرایط عدم تنش بدست آمد. نتایج نیز نشان داد که بیشترین میزان کلروفیل کل شنبلیله در شرایط عدم تنش خشکی و درتیمارهای کودی 5 کیلوگرم بیوپتاس و سولفات آهن 5/1 درهزار بدست آمد. بر اساس نتایج این آزمایش، استفاده از تنش خشکی، نقش موثری در افزایش محتوی فنلی، فلاونییدها، آنتوسیانین و قند‌های محلول شنبلیله داشت و همچنین کاربرد کود زیستی بیوپتاس و سولفات آهن، صفات بیوشیمیایی شنبلیله را بهبود بخشید.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of antioxidant activities and biochemical characteristics of drought-stressed fenugreek (Trigonella foenum- graecum L.) in response to biopotasse and iron sulfate

نویسندگان [English]

  • Mohammed Hossein Aminifard 1
  • homeyra. homeyra 2
  • Hassan bayat 1
  • Ali Reza Samadzade 3
1 Department of Horticultural Science and Special Plants Regional Research Center, College of Agriculture, University of Birjand, Birjand, Iran
2 Department of Horticultural Science, College of Agriculture, University of Birjand, Birjand, Iran
3 Department of Agronomy and Plant Breeding Sciences, College of Agriculture, University of Birjand, Birjand, Iran
چکیده [English]

In order to investigate the effect of biopotasse and iron sulfate on antioxidant activity and biochemical characteristics of fenugreek under drought stress conditions, a factorial experiment was conducted as split plot based on randomized complete block design with three replications in 2017. Treatments included drought stress (normal irrigation as non-stress and irrigation in early growth for 14 days as drought stress) as the main factor and biopotasse (0 and 5 kg. ha-1) and iron sulfate (0 and 1.5 per thousand) as sub-factors biofertilizer. Results showed that the effect of drought stress, biopotasse, iron sulfate, and their interaction were significant on anthocyanin and phenolic compounds and flavonoids so that the highest amount of anthocyanin in fenugreek was obtained from 5 kg biopotasse treatment per hectare and 1.5 per thousand iron sulfate in drought stress conditions while the lowest amount was recorded in control treatment under stress conditions. Also, in drought stress, phenolic compounds, and flavonoids increased in fenugreek, as the highest amount of total flavonoid was achieved under drought stress conditions and 5 kg ha-1 biopotasse and 1.5 per thousand iron sulfates. The interaction of drought stress and iron sulfate was significant on the antioxidant activity of the plant, as the highest antioxidant activity (62.8%) was obtained in drought stress and iron sulfate treatment. Also, the highest amount of total carbohydrate in fenugreek was obtained under drought stress conditions and in 5 kg biopotasse treatment, and the lowest trait was obtained under non-stress conditions. Results showed that the highest total chlorophyll content of fenugreek was observed in non-drought stress conditions and 5kg .ha-1 biopotasse and iron sulfate 1.5 per thousand. Based on the findings, drought stress played an important role in increasing phenolic compounds, flavonoids, anthocyanin, and soluble sugar contents of fenugreek and biopotasse biofertilizer and iron sulfate improved biochemical characteristics of the plants.

کلیدواژه‌ها [English]

  • Biochemical characteristics
  • Carbohydrate
  • Fenugreek
  • Secondary metabolites
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