مقایسه اثر سیلیکون معدنی و نانوسیلیکون بر برخی صفات بیوشیمیایی و فتوسنتزی در گیاه ذرت Zea mays L.تحت تنش شوری

نوع مقاله: پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم، دانشگاه گلستان، گرگان

2 استاد گروه زیست شناسی، دانشکده علوم، دانشگاه گلستان

3 گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

4 گروه علوم پایه، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

تنش شوری یکی از عواملی است که رشد و عملکرد ذرت را تهدید می‌کند. اثرات کاربرد سیلیکون معدنی و یا نانوسیلیکون در تخفیف شوری در برخی گیاهان گزارش شده است. به این منظور اثرات تغذیه برگی سیلیکون معدنی و نانو در تخفیف اثرات تنش شوری در رشد و برخی عوامل بیوشیمایی و فتوسنتزی در گیاه ذرت مورد بررسی و مقایسه قرار گرفت. آزمایشات در قالب طرح کاملاً تصادفی و در قالب فاکتوریل به‌صورت کشت گلدانی انجام شد. فاکتور اول شوری در دو سطح 0 و 100 میلی‌مولار کلریدسدیم و سیلیکون در سطوح شاهد (بدون سیلیکون)، 2 میلی‌مولار سیلیکات پتاسیم و 2 میلی‌مولار نانوسیلیکون SiO2 بود. تحت تنش شوری، میزان وزن تر و خشک بخش هوایی و ریشه، میزان کلروفیل‌ها و کاروتنوئیدها و میزان پروتئین‌های محلول نسبت به شاهد به صورت معنی‌داری کاهش و در مقابل میزان پراکسیدهیدروژن و مالون دی‌آلدهید این گیاهان افزایش یافت. همچنین، تنش شوری میزان فتوسنتز و کارآیی مصرف آب گیاهان را کاهش داد. کاربرد سیلیکون و نانوسیلیکون رشد گیاهان تحت شوری را بهبود بخشید و موجب افزایش شدت فتوسنتز و میزان رنگدانه‌های فتوسنتزی در آنها شد. به علاوه، کاهش شدید تعرق با کاربرد سیلیکون نسبت به نانوسیلیکون سبب افزایش بیشتر راندمان مصرف آب در این تیمار شد. همچنین کاربرد سیلیکون فعالیت آنزیم پراکسیداز محلول را در گیاهان تحت شوری افزایش داد که با کاربرد نانوسیلیکون مشاهده نشد. این امر کاهش بیشتر پراکسیدهیدروژن و پراکسیداسیون لیپید گیاهان تحت شوری با تیمار سیلیکون نسبت به نانوسیلیکون را به دنبال داشت که کاهش بیشتر تنش اکسیدایتو در این تیمار را به نمایش گذاشت این نتایج نشان داد که تخفیف تنش و افزایش رشد گیاهان تحت شوری با کاربرد سیلیکون نسبت به نانوسیلیکون بیشتر بود که احتمالاً به کاهش بیشتر تنش اکسیداتیو در این تیمار مربوط است.
 

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of the effect of silicon and nano-silicon on some biochemical and photosynthetic traits of Zea mays L. under salinity stress

نویسندگان [English]

  • Mahbobeh Zarooshan 1
  • Ahmad Abdilzade 2
  • Hamid Reza Sadeghipour 3
  • Pooyan Mehrabanjoubani 4
1 Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
2 Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
3 Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
4 Department of Basic Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

Salinity stress is one of the factors that threatens the growth and yield of corn. The effects of silicon or nanosilicon on salinity alleviation have been reported in some plants. The effects of foliar application of silicon (inorganic and nanopatricle) on mitigation of the effects of salinity stress on some photosynthetic and biochemical parameters were studied in corn. Experiments were conducted in a completely randomized design with factorial arrangement in a pot culture. The first factor was salinity at two levels including 0 and 100 mM NaCl and the second factor was silicon at three levels including control (without silicon) and 2 mM potassium silicate and 2 mM nanosilicon (SiO2). Under salinity stress, the fresh and dry weights of shoots and roots, the chlorophylls and carotenoids contents, and the amount of soluble protein decreased significantly compared to the control. In contrast, the amount of hydrogen peroxide and malondialdehyde increased in these plants. Also, the photosyntheic rate and water use efficiency of plants decreased under salinity. The application of silicon and nanosilicon improved the growth of plants under salinity and increased the photosynthetic rate and the amount of photosynthetic pigments. In addition, severe reduction in transpiration under silicon application compared to nanosilicon resulted in the increased water use efficiency in this treatment. Silicon application also increased the activity of soluble peroxidase enzyme in plants under salinity, a result which was not observed with nanosilicon application. This resulted in a further decrease in hydrogen peroxide and lipid peroxidation of plants under salinity treated with silicon compared to nanosilicon, which showed a further decrease in oxidative stress in this treatment. These findings indicated that stress reduction and growth improvement of the plants under salinity with silicon application were more than the treatment with nanosilicon.

کلیدواژه‌ها [English]

  • NaCl stress
  • Nanosilicon
  • Oxidative stress
  • Photosynthesis
  • silicon
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