ارزیابی فعالیت آنتی اکسیدانتی و کاربرد تکنیک HPLC جهت آنالیز ترکیبات فنلی عصاره برگ درخت بادام زاگرسی (Amygdalus haussknechtii L.)

نوع مقاله: پژوهشی

نویسندگان

1 عضو هیات علمی گروه زیست‌شناسی، دانشکده علوم، دانشگاه ایلام

2 گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه ایلام، ایلام، ایران

چکیده

این تحقیق با هدف بررسی و مقایسه  میزان ترکیبات آنتی اکسیدانتی و فعالیت آنتی اکسیدانتی سه ژنوتیپ بادام زاگرسی در رویشگاه­های طبیعی استان ایلام انجام گرفت. محتوی فنل کل با استفاده از روش فولین- سیکالتئو و فعالیت آنتی اکسیدانتی با استفاده از دو سنجش ظرفیت جمع­آوری رادیکال DPPH و ظرفیت جمع‌آوری رادیکال نیتریت ارزیابی شد. جهت تعیین کمی ترکیبات فنلی از دستگاه کروماتوگرافی مایع با کارایی بالا استفاده گردید. نتایج نشان داد که فعالیت پاداکسایشی عصاره برگ ژنوتیپ H3 به­طور معنی­داری بیشتر از ژنوتیپ­های H1 و H2 بوده که این فعالیت بالا را می­توان به میزان بالای محتوی فنلی و فلاوونوئیدی نسبت داد، به­طوری­که همبستگی بالایی بین محتوی فنل کل با ظرفیت جمع­آوری رادیکال DPPH (902/0 =R) و ظرفیت جمع­آوری رادیکال نیتریت (806/0 =R) مشاهده شد. آنالیز ترکیبات فنلی توسط HPLC وجود ترکیبات کاتچین، سیناپیک اسید، کوئرستین و آپیژنین در ژنوتیپ H3 را تایید نمود. همچنین ترکیب آپژنین در هر سه ژنوتیپ مورد مطالعه مشاهده شد. به­طور­کلی نتایج پیشنهاد می­دهند که عصاره برگ ژنوتیپ H3 با محتوی بالایی از ترکیبات فنلی و فلاوونوئیدی و فعالیت پاداکسایشی بالا می­تواند به‌عنوان منبعی از ترکیبات پاداکسایشی در صنعت داروسازی مورد استفاده قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of antioxidant activity and application of HPLC technique for analysis of phenolic compounds of Amygdalus haussknechtii leaf extract

نویسندگان [English]

  • Ali Asghar Hatamnia 1
  • Rabie Sharifi 2
1 Department of Biology, Faculty of Science, Ilam University, Ilam, Iran
2 Department of Biology, Faculty of Science, Ilam University, Ilam, Iran
چکیده [English]

This study was conducted to investigate antioxidant compounds contents and antioxidant activity of three Amygdalus haussknechtii genotypes in the natural habitat of Ilam Province. Total phenolic content was measured using Folin–Ciocalteu method and antioxidant activity was estimated by two methods, namely DPPH (2,2-diphenyl- 1- picrylhydrazyl) and nitric oxide radical scavenging. High- performance chromatography was used for quantitative estimation of the phenolic compounds. Results showed that antioxidant activity of leaf extract of H3 genotype was significantly higher than that of H1 and H2 genotypes. The high antioxidant activity can be attributed to higher total phenolic and flavonoid contents, so that high correlation coefficient was observed between total phenolic content and DPPH and nitric oxide radical scavenging, R = 0.902 and R= 0.806, respectively. Analyzing the phenolic compounds by HPLC method indicated that catechin, sinapic acid, quercetin, and apigenin were found in H3 genotype. Also, apigenin compound was observe in all three investigated genotypes. Generally, the results suggested that leaf extract of H3 genotype with high phenolic and flavonoid content and antioxidant activity can be presented as a source of natural antioxidant and used in pharmaceutical industries.

کلیدواژه‌ها [English]

  • Amygdalus Haussknechtii
  • antioxidant activity
  • HPLC
  • leaf
  • phenolic compounds
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