بررسی جوانه‌زنی بذر و تغییرات فیتوشیمیایی دو توده گل گاوزبان ایرانی (Echium amuenum Fisch & C.A.Mey) تحت تاثیر منطقه کشت

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشگاه آزاد اسلامی، واحد تهران شمال، باشگاه پژوهشگران جوان و نخبگان، تهران، ایران

چکیده

گل گاو زبان (Echium amoenum) از مهمترین گیاهان تیره Boragicaceae است. به منظور تعیین مناسب‌ترین روش برای شکستن خواب بذر، تحقیق حاضر در قالب دو آزمایش انجام شد. آزمایش اول با بذور دو جمعیت­ گل گاوزبان جمع آوری شده از مشهد و جواهرده در قالب آزمایش فاکتوریل با 3 تکرار انجام شد. تیمارهای پیش رویشی استفاده شده در این تحقیق عبارتنداز: تیمار بذور مرطوب شده در دمای 4 درجه سانتی‌گراد به‌مدت ۷، ۱۴ و ۲۱ روز متوالی در دمای یخچال، تیمار بذور با اسید جیبرلیک 500 پی­پی­ام به مدت ۲۴ ساعت و تیمار بذور با اسید سولفوریک ۵۰ درصد به مدت ۱۰ دقیقه. آزمایش دوم در قالب طرح بلوکهای کامل تصادفی در مکان و زمان در دو فصل زراعی با چهار تکرار، با استفاده از دو توده به صورت همزمان در گرگان و مشهد کشت شد تا اثر اقلیم بر خصوصیات کمی و کیفی گل مورد بررسی قرار گیرد. نتایج نشان داد که خواب بذر گل گاوزبان ایرانی از نوع فیزیولوژیک است، زیرا بیشترین درصد جوانه­زنی بذرها در اثر اعمال تیمار پیش سرمای مرطوب در ۷ روز به دست آمد. مقایسه توده­های مذکور در دو منطقه نشان داد که  عملکرد گل و میزان متابولیت­های ثانویه (آنتوسیانین، فعالیت آنتی­اکسیدانی، فنل و فلاونوئید) گیاهان کشت شده در مشهد نسبت به گرگان مطلوب­تر بود. لذا با توجه به مقاومت نسبی گیاه به کم آب پیشنهاد می­گردد که گسترش کشت این گیاه در خراسان رضوی مورد ارزیابی بیشتری قرار گیرد. همچنین پیش تیمار 7 روز سرمادهی مرطوب برای تسهیل و یکنواختی جوانه زنی پیشنهاد می­‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Seed germination and phytochemical variations of two Iranian borage (Echium amuenum Fisch & C.A.Mey) under the influence of cultivation location

نویسندگان [English]

  • Manijeh Khorsandi Aghaii 1
  • Azim Ghasemnezhad 1
  • javad Mousavizade 1
  • Esmaeil Babakhanzadeh sajirani 2
1 Department of Horticulture, Faculty of Plant Production, University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Young and Elite Research Club, Tehran North Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Iranianborage (Echium amoenum) is one of the most important plants of Boragicaceae. In order to determine the most suitable method of overcoming seed dormancy of this plant, the present study was conducted in two different experiments. The first experiment was carried out using seeds collected from Mashhad and Javaherdeh based on a factorial study with three replications. Pretreatments were seed treatment at 4° C for 7, 14, and 21 consecutive days under refrigerated temperature, seed treatment with 500 ppm gibberellic acid for 24 hours, and seed treatment with 50% sulfuric acid for 10 minutes. The second experiment was conducted in a randomized complete block design in time and location  in two years  and  two locations  of Gorgan and Mashhad. The experiment was carried out to evaluate the effect of climate on quantitative and qualitative characteristics of the flower. Results showed that Iranian borage seed has physiological dormancy since the maximum seed germination percentage was obtained by applying pre-cooling treatments in 7 days. Comparison of the two locations revealed more favorable flower yield and secondary metabolite accumulation (anthocyanin, antioxidant, phenol and flavonoid) in plants cultivated in Mashhad region. Therefore, due to the relative resistance of the plant to water deficit, the cultivation of this plant in Mashhad is suggested to be further evaluated. Also, pre-treatment of 7 days of moist chilling is also recommended to facilitate the germination uniformity.

کلیدواژه‌ها [English]

  • Anthocyanin
  • antioxidant activity
  • Iranian borage
  • Medicinal Plants
  • Physiological sleep
  • Seed germination
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