بررسی اثر هورمون‌های سیتوکینین و جیبرلین بر ویژگی‌های جوانه‌زنی و فعالیت آنزیم‌‌های‌ آنتی‌اکسیدانی در بذور ارقام ذرت زوال یافته (Zea mays L.)

نوع مقاله: پژوهشی

نویسنده

گروه کشاورزی ، دانشگاه پیام نور، تهران ، ایران

چکیده

زوال بذر ‌یکی از عوامل کاهش دهنده بنیه و محدودکننده جوانه‌زنی در ‌بذر می‌باشد. شناسایی عوامل تاثیر گذار بر زوال بذر بسیار با اهمیت است. به‌منظور بررسی اثر هورمون­های گیاهی بر ویژگی­های جوانه‌زنی و فعالیت آنزیم‌های آنتی اکسیدانت بذور فرسوده ذرت آزمایشی در سال 1398 در آزمایشگاه بذر گروه زراعت و اصلاح نباتات دانشکده علوم زراعی و دامی دانشگاه تهران انجام گرفت. این تحقیق به‌صورت آزمایش فاکتوریل در قالب طرح کاملا تصادفی با چهار تکرار انجام شد. تیمارهای آزمایش شامل عامل رقم در دو سطح (رقم 704 و رقم 260)، عامل پیری زودرس در 4 سطح (0، 5، 10 و 15 روز) و عامل هورمون در دو سطح (هورمون جیبرلین و هورمون سیتوکینین) بودند. زوال بذر سبب کاهش شاخص­های جوانه­زنی و کاهش فعالیت آنزیم­های آنتی اکسیدانت پراکسیداز و کاتالاز شد. تیمار بذرهای زوال یافته با هورمون­های سیتوکینین و جیبرلین سبب افزایش فعالیت آنزیم‌های آنتی اکسیدان شد. هورمون سیتوکینین نسبت به هورمون جیبرلین اثربیشتری بر افزایش فعالیت آنزیم‌های آنتی اکسیدان در بذرهای زوال یافته داشت. نتایج نشان داد که بنیه ضعیف بذر سبب کاهش فعالیت آنزیم­ها می­شود و هورمون­های گیاهی می تواند به عنوان یک رهیافت زراعی سبب جبران خسارت در بذرهای با بنیه پایین ‌استفاده شود. همچنین طبق نتایج بدست آمده رقم 704 با بنیه بالاتر فعالیت آنزیمی بیشتری را نسبت به رقم 260 نشان ‌داد.

کلیدواژه‌ها


عنوان مقاله [English]

The study of the effects of cytokinin and gibberellin on germination characteristics and antioxidant enzymes activity in deteriorated seeds of corn cultivars (Zea mays L.)

نویسنده [English]

  • saedeh rashidy
Agronomy Department, Payame Noor University, Tehran, Iran
چکیده [English]

Deterioration of seed is one of the vigor reducing factors limiting seed germination. Determination of factors affecting seed deterioration is important. In order to determine the effect of plant hormones on germination characteristics and antioxidant enzymes activities of deteriorated corn seeds an experiment was carried out at seed and biotechnology laboratories of College of Agriculture, Tehran University in 2019. The study was designed with a factorial experiment based on completely randomized design (CRD) with four replications. The experiment treatments included varieties at 2 levels (704 single cross hybrid and 260 single cross hybrid), accelerated aging at 4 levels (0, 5, 10, and 15 day), and hormones at 2 levels (gibberellin and cytokinin). Seed deterioration reduced germination indexes and peroxidase and catalase enzymes activity. Treatment of deteriorated seeds with cytokinin and gibberellin increased antioxidant enzymes activities. Cytokinin was more effective than gibberellin in increasing the activities of antioxidant enzymes in deteriorated seeds. Results indicated that low seed vigor decreased the activity of enzymes and plant hormones as an agronomy solution compensated for the loss in low vigor seeds. Also, cultivar 704, with a higher vigor indicated amore enzyme activity than cultivar260.
 

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • corn
  • Cytokinin
  • Gibberellin
  • Seed deterioration
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