بررسی برخی پاسخ‌های مورفولوژیکی و فیزیولوژیکی گیاه دارویی بادرشبویه (Dracocephalum moldavica L.) به سلنیوم تحت تنش شوری

نوع مقاله: پژوهشی

نویسندگان

گروه زیست‌شناسی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

شوری یکی از مهم‌ترین تهدیدهای محیطی است که بر رشد، توسعه و بهره‌وری گیاهان اثر می‌گذارد، لذا دسترسی به روش‌های کاهش اثرات منفی آن بر روی گیاهان  امری ضروری  به نظر می رسد. به همین منظور، آزمایشی گلخانه‌ای در قالب طرح کاملاً تصادفی با تیمارهای شوری (0، 25، 50 و 75 میلی‌مولار) و محلول‌پاشی سلنیوم (0، 5، 10 میکرومولار) اجرا گردید تا نقش احتمالی سلنیوم در تنظیم بردباری گیاه دارویی بادرشبویه به شوری مورد بررسی قرار گیرد. در این راستا محلول‌پاشی‌ پس از استقرار گیاهچه‌ها در سه مرحله زمانی، هم‌زمان با اعمال تنش شوری و سپس به فاصله هر هفته یک‌بار انجام شد. نتایج نشان داد که شاخص‌های رشد، رنگیزه‌های فتوسنتزی و محتوای نسبی آب برگ گیاهان بادرشبویه تحت تأثیر تنش شوری در سطوح مختلف به‌طور معنی‌دار کاهش پیدا کردند. محلول‌پاشی سلنیوم به‌ویژه در غلظت کم سبب بهبود کلیه صفات ذکرشده در شرایط تنش شوری گردید. در مقابل، سطح بالای سلنیوم سبب تشدید اثر منفی شوری شد، به‌طوری‌که حداکثر میزان مالون‌دی‌آلدئید، نشت الکترولیتی و پتانسیل‌آب توسط سلنیوم (10 میکرومولار) در شرایط تنش شوری شدید (75 میلی‌مولار) حاصل گردید و منجر به تجمع پرولین و قندهای محلول شد. این بررسی نشان داد  تیمار سلنیوم در غلظت پایین نقش بسزایی در کاهش اثرات مضر شوری از طریق افزایش رنگیزه‌های فتوسنتزی، حفظ یکپارچگی غشاء، کاهش پتانسیل‌آب و انباشت محلول‌های سازگار داشته و در نتیجه سبب بهبود عملکرد گیاهان بادرشبویه تحت تنش شوری شد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of some morphological and physiological responses of Moldavian balm (Dracocephalum moldavica L.) to selenium under salinity

نویسندگان [English]

  • Alireza Iranbakhsh
  • Sara Saadatmand
  • Ramazanali Khavarinejad
  • Bita Zaji
Department of Biology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Salt stress is one of the most important environmental threats that adversely affects the growth, development, and productivity of plants; therefore, suitable approaches are needed to mitigate its negative effects on plants. For this purpose, a pot experiment was carried out in a completely randomized design with two treatments include salinity (0, 25, 50, and 75 mM) and foliar application of selenium (0, 5 and 10 µM Na2SeO4), and the possible role of selenium in regulating Moldavian balm salt tolerance was investigated. Foliar applications of selenium were applied after seedlings establishment at three stages, simultaneously with the application of salinity stress, and then at weekly intervals. Results indicated that growth indices, photosynthetic pigments, and relative water content of leaves in Moldavian balm plants were decreased significantly by various levels of salinity stress. Foliar application of selenium especially at the low concentration increased all the mentioned traits in stress conditions. In contrast, high level of selenium magnified the negative effect of salinity, so that the highest value of malondialdehyde, electrolyte leakage, and water potential were obtained by 10 µM selenium under 75 mM NaCl and caused the accumulation of proline and soluble sugars. This study indicates that selenium at low concentration plays a significant role in alleviating the harmful effects of salinity through photosynthetic pigments, maintaining membrane integrity, reducing water potential, accumulation of compatible solutes, and consequently, improved the performance in Moldavian balm plants growing under salt stress.

کلیدواژه‌ها [English]

  • water potential
  • Photosynthetic pigments
  • selenium
  • Malondialdehyde
  • Compatible solutes
  • Electrolyte leakage
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