تاثیر پیش تیمار اسپرمیدین و پلی‌اتیلن گلیکول بر جوانه‌زنی و برخی فعالیت‌های فیزیولوژی و مرفولوژی در گیاه گندم دروم (Triticum durum)

نوع مقاله: پژوهشی

نویسنده

گروه علوم گیاهی، دانشکده زیست‌شناسی، دانشگاه آزاد اسلامی واحد تنکابن، تنکابن، ایران

چکیده

به‌منظور بررسی تاثیر پیش ‌تیمار اسپرمیدین و پلی‌اتیلن‌گلیکول بر روی جوانه‌زنی و برخی از شاخص‌های فیزیولوژی و مورفولوژی در گیاه گندم تحت تنش‌ خشکی، یک آزمایش فاکتوریل در قالب بلوک‌های کامل تصادفی با 20‌ تیمار و سه‌ تکرار اجرا شد. تیمارهای آزمایشی شامل پنج سطح اسپرمیدین (0، 02/0 ، 05/0، 1/0و 15/0 میلی‌مولار) و چهار سطح پلی‌اتیلن‌گلیکول (0، 2-، 4- و 5- بار) بودند. نتایج آزمایشات نشان داد که کاربرد اسپرمیدین باعث افزایش وزن نسبی برگ، سطح برگ، کلروفیل ،کربوهیدرات و فنل کل گردید. در این مطالعه با افزایش سطح اسپرمیدین از میزان کاروتنوئیدها و ظرفیت انتی اکسیدانی برگ کاسته شد. همچنین بر روی جوانه زنی به لحاظ آماری اثر معنی داری نداشت. تنش خشکی که با پلی‌اتیلن‌گلیکول مورد ارزیابی قرار گرفت. باعث افزایش ظرفیت آنتی‌اکسیدانی، فنل کل وکربوهیدرات گردید. نتایج نشان داد که افرایش سطح پلی‌اتیلن‌گلیکول منجر به کاهش جوانه‌زنی برگ شد. بنابراین با توجه به نتایج فوق بکارگیری اسپرمیدین و پلی اتیلن گلیکول بعنوان پیش تیمار بذر می تواند در شرایط تنش برروی شاخص‌های فیزیولوژیکی ومورفولوژیکی تاثیر قابل توجهی داشته باشد و توان گیاه را در شرایط تنش افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of premixing of spermidine and polyethylene glycol on germination and physiological and morphological activity in Triticum durum wheat with Salinity stress

نویسنده [English]

  • Parvane Rahdari
Department of Plant Physiology, Islamic Azad University, Tonekabon Branch, Tonekabon, Iran
چکیده [English]

In order to effect of spermidine and polyethylene glycol pretreatment on germination and some physiological and morphological activity durum wheat under salinity stress a  experiment with 20 treatments, three replications was conducted in 60 experimental units. Treatments included five levels of spermidine (0/ 02,0/ 05,0/ 1 and 0/15 mM) and four levels of polyethylene glycol (0- 2,- 4 and -5 bar). The results showed that the use of spermidine increased the relative weight contact of leaf (RWC), leaf area ratio, chlorophyll s and phenolic compounds . In this study, carotenoids content and leaf antioxidant activity decreased with increasing spermidine level and also had no statistically significant effect on germination. With increase of polyetylen glycol antioxidant activity, carbohydrates and  total phenolics increased  but decreased germination. Therefore, according to the above results, application of spermidine and polyethylene glycol as seed pre-treatments can have significant effects on physiological and morphological activities in stress conditions and increase plant potency under stress conditions.   

کلیدواژه‌ها [English]

  • Drought stress
  • Polyethylene glycol
  • Physiological factors
  • Spermidine
  • Triticum durum
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