بررسی اثر ورمیکمپوست و تنش شوری بر میزان رنگیزه‌ها و برخی صفات بیوشیمیایی گیاه گاوزبان اروپایی (Borago officinalis L.)

نوع مقاله: پژوهشی

نویسندگان

1 استادیار فیزیولوژی گیاهی، واحد کلیبر، دانشگاه آزاد اسلامی کلیبر، ایران

2 دانشجوی کارشناسی ارشد زراعت، واحد کلیبر، دانشگاه آزاد اسلامی، کلیبر، ایران

چکیده

به‌منظور بررسی اثرات شوری و ورمی‌کمپوست بر میزان فعالیت آنزیم‌های آنتی‌کسیدان و رنگیزه‌های فتوسنتزی گیاه گاوزبان اروپایی (Borago officinalis L.) آزمایشی به‌صورت فاکتوریل در‌ قالب طرح کاملا تصادفی با چهار تکرار در سال 1394 اجرا شد. تیمارها شامل چهار سطح ورمی‌کمپوست (صفر، 5، 10 و 15 درصد وزنی-وزنی) و چهار سطح شوری (صفر (شاهد)، 4، 8 و 12 دسی‌زیمنس بر متر کلریدسدیم) بود. نتایج حاصل از تجزیه واریانس نشان داد که اثر متقابل تنش شوری و ورمی‌کمپوست بر میزان فعالیت آنزیم‌های سوپراکسید دیسموتاز، آسکوربات پراکسیداز، گلوتاتیون پراکسیداز، کاتالاز و کلروفیل کل معنی‌د‌ار بود. سایر نتایج مشخص ساخت که با افزایش شوری میزان فعالیت آنزیم‌های آنتی‌اکسیدان افزایش و مقادیر رنگیزه‌های فتوسنتزی کاهش یافت. همچنین استفاده از کود ورمی‌کمپوست نسبت به شاهد به‌طور معنی‌داری میزان کلروفیل a، کلروفیل  bو کاروتنوئیدها را افزایش داد. نتایج مقایسه میانگین برهمکنش تنش شوری و ورمی‌کمپوست نشان داد که حداکثر میزان فعالیت آنزیم‌های آنتی‌اکسیدان توسط تیمار 15% وزنی ورمی‌کمپوست در سطح شوری 12 دسی‌زیمنس بر متر کلرید‌سدیم حاصل شد. بنابراین، استفاده از ورمی‌کمپوست به‌عنوان یک کود آلی، علاوه بر افزایش میزان فعالیت آنزیم‌های آنتی‌اکسیدان و مقادیر رنگیزه‌های فتوسنتزی، می‌تواند راهکار مناسبی برای کاهش اثرهای منفی ناشی از غلظت زیاد یون‌های سدیم و کلر در خاک‌ها بر رشد گاوزبان اروپایی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

vEvaluation of the effect of vermicompost and salinity stress on the pigments content and some biochemical characteristics of Borage (Borago Officinalis L.)

نویسندگان [English]

  • Ahmad Afkari 1
  • Parvin Farajpour 2
1 2- Assist. Prof., Dept. of Physiology, Kaleybar Branch, Islamic Azad University Kaleybar, Iran
2 2- Master of Agronomy Graduate, Kaleybar Branch, Islamic Azad University, Kaleybar, Iran
چکیده [English]

In order to investigate the effects of salinity and vermicompost on the antioxidant enzymes activity and photosynthetic pigments of Borage officinalis L., a factorial experiment was carried out in a completely randomized design with four replications in 2015. The study treatments consist of four vermicompost levels (0, 5, 10, and 15% wt potted soil in dry weight) and four salinity levels 0 (control), 4, 8, and 12 ds/m sodium chloride. The results of analysis of variance showed a considerable influence of salinity stress and vermicompost on the activity level of superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, catalase, and total chlorophyll enzymes. Other results indicated that with increasing salinity, the activity level of antioxidant enzymes and photosynthetic pigments increased and decreased, respectively. Also, use of vermicompost fertilizer raised the levels of chlorophyll a, chlorophyll b, and carotenoids significantly compared to the control. Comparison of means of the interactions between salinity stress and vermicompost showed that the maximum antioxidant enzymes activity was obtained in 15 wt % vermicompost treatment at the salinity level of 12 ds/m NaCl. Therefore, the use of vermicompost as an organic fertilizer, in addition to increasing the activity of antioxidant enzymes and photosynthetic pigments, can be a good way to reduce the negative effects of high levels of sodium and chloride ions in soils on the growth of borage.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • Borago officinalis L
  • carotenoids
  • Chlorophyll
  • Sodium chloride
  • vermicompost
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