اثر نانولوله های کربنی چند جداره بر تحریک ریشه‌زایی قلمه‌های به‌لیمو (Lippia citriodora L.)

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شیراز، شیراز، ایران

2 مرکز تحقیقات فرآوری گیاهان دارویی، دانشگاه علوم پزشکی شیراز، شیراز، ایران

3 گروه تحقیقات منابع طبیعی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی فارس، سازمان تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایران

چکیده

امروزه استفاده از نانولوله­های کربن در علوم گیاهی جایگاه ویژه­ای پیدا کرده و اثرات قابل قبولی از آن گزارش شده است. به‌منظور مطالعه اثر نانولوله­های کربنی چند­جداره (0، 10، 25، 50، 75 و 100 میلی­گرم در لیتر) بر تحریک ریشه‌زایی قلمه‌های به‌لیمو (Lippia citriodora L.)، آزمایشی گلدانی به‌صورت فاکتوریل در قالب طرح بلوک کامل تصادفی با 4 تکرار در مزرعه تحقیقاتی دانشگاه شیراز انجام شد. نتایج نشان داد کاربرد نانولوله­های کربنی چند جداره در غلظت‌های 10 تا 50 میلی­گرم در لیتر به‌طور معنی‌داری منجر به بهبود ویژگی‌های مورفولوژیکی ریشه در مقایسه با شاهد شد. بیشترین افزایش در وزن ریشه (حدود 200% افزایش نسبت به تیمار شاهد) در پاسخ به کاربرد 50 میلی­گرم در لیتر نانولوله­های کربن مشاهده شد. همچنین حجم ریشه در پاسخ به این غلظت از تیمار تا حدود 165% نسبت به شاهد ارتقاء یافت؛ درحالی‌که کاربرد 100 میلی‌گرم در لیتر نانولوله­های کربنی حجم ریشه را به‌طور معنی‌داری کاهش داد. پایین‌ترین تعداد ریشه‌ها در قلمه‌های تیمار شده با 100 میلی­گرم در لیتر نانولوله­های کربن مشاهده شد. اعمال نانولوله­های کربنی به میزان 50 میلی­گرم در لیتر تعداد ریشه‌های موجود در قلمه‌ها را به‌طور معنی‌داری (تا 50 درصد) نسبت به تیمار شاهد افزایش داد. از سوی دیگر، بالاترین طول ریشه در نمونه‌ها به­دنبال اعمال این غلظت از تیمار مشاهده شد بطوری­که 94/65 درصد افزایش نسبت به شاهد ثبت شد. تحقیق حاضر ضمن گزارش اثرات امید­بخش نانولوله‌های کربنی در ارتقاء ریشه­زایی گونه به­لیمو، مطالعات تکمیلی در رابطه با بهبود رشد اندام­های بالازمینی این گونه دارویی را پیشنهاد می­ کند.

کلیدواژه‌ها


عنوان مقاله [English]

Influence of multi-walled carbon nanotubes (MWCNTs) on rooting of cuttings in Lippia citriodora L.

نویسندگان [English]

  • Zabihollah Rigi Karvandari 1
  • Mohammad Jamal Saharkhiz 2
  • Fatemeh Raouf fard 1
  • Mehrdad Zarafshar 3
1 Department of Horticultural Sciences, Faculty of Agriculture, Shiraz University, Shiraz, Iran
2 Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran
چکیده [English]

Nowadays, carbon nanotubes (CNTs) have a special place in plant science and promising effects have been reported. To study the effects of multi-walled carbon nanotube (MWCNTs), 0 (control), 10, 25, 50, 75 and 100 mg L-1, on rooting of cuttings in Lippia citriodora L., a factorial pot experiment in the form of randomized complete block design was set up in the research field of Shiraz University with 4 replications. Results showed that the application of multi-walled carbon nanotubes (10-50 mg L-1) significantly improved the root morphological traits compared to the control treatment. The highest increase in root weight (around 200% compared with control) was recorded in response to 50 mg L-1 MWCNT treatment. The root volume also increased by 165% under 50 mg L-1 MWCNT treatment while the lowest value was observed in cuttings treated with 100 mg L-1MWCNT. The lowest number of roots in cuttings belonged to 100 mg L-1MWCNT treatment. Application of 50 ppm MWCNT in comparison with control treatment significantly increased the number of roots in cuttings (by 50%). The application of this concentration caused the highest amount of root length which was 65.94% more than the control treatment. Although our finding showed promising effects of MWCNT on root of Lippia citriodora, the further comprehensive studies can are suggested on shoot biomass of the herbal plant.

کلیدواژه‌ها [English]

  • Morphology
  • Dry biomass
  • Root
  • Nano technology
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