تاثیر قارچ‏های مایکوریزا و محلول‌پاشی پوترسین بر صفات بیوشیمیایی و بیوماس اندام هوایی ریحان سبز (Ocimum ciliatum L.) در دو چین مختلف برداشت

نوع مقاله: پژوهشی

نویسندگان

گروه علوم باغبانی و مهندسی فضای سبز، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

به‌منظور بررسی تاثیر قارچ‏های مایکوریزا و محلول‏پاشی پوترسین بر برخی صفات بیوشیمیایی، میزان اسانس و بیوماس اندام هوایی ریحان سبز (Ocimum ciliatum L.)، آزمایشی گلدانی به‌صورت اسپلیت پلات در زمان در قالب طرح کاملاً تصادفی با سه تکرار انجام شد. فاکتورهای آزمایش شامل قارچ مایکوریزا در 3 سطح (عدم کاربرد، Glomus mossea، Glomus intraradicese) و محلول­پاشی پوترسین در سه سطح (0، 1 و 2 میلی‏مولار) به‌عنوان فاکتورهای اصلی و چین برداشت به عنوان فاکتور فرعی در نظر گرفته شدند. نتایج نشان داد کاربرد قارچ‏های مایکوریزا و پوترسین بر صفات بیوشیمیایی، بیوماس تر و خشک اندام هوایی و میزان اسانس گیاه تاثیر داشتند. بیشترین میزان کلروفیل a  و میزان اسانس در تیمار کاربرد قارچ G. mossea و پوترسین با غلظت 2 میلی‏مولار در چین اول مشاهده شدند. بیشترین میزان کلروفیل b و کارتنوئیدها با کاربرد قارچ
 G. intraradicese و پوترسین با غلظت 2 میلی‏مولار در چین اول بدست آمد. اما با افزایش طول روز و دما در چین دوم برداشت از میزان رنگیزه‏های فتوسنتزی کاسته شد. بیشترین فعالیت آنتی‏اکسیدانی و فنل کل در تیمار کاربرد قارچ G. intraradicese و عدم محلول­پاشی پوترسین در چین دوم برداشت مشاهده شد. بیوماس تر و خشک اندام هوایی با کاربرد قارچ G. mossea به بیشترین میزان خود رسید؛ اما تفاوت معنی‏داری بین دو غلظت پوترسین مشاهده نشد و تنها نسبت به عدم کاربرد پوترسین افزایش معنی‏داری نشان داد. به‌طورکلی کاربرد هر دو نوع قارچ به عنوان کود زیستی همراه با محلول‏پاشی پوترسین در غلظت 2 میلی‏مولار توانست صفات مورد مطالعه در این تحقیق را به‌طور معنی‏داری بهبود بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of mycorrhizal fungi and foliar application of putrescine on some biochemical characteristics and biomass of basil (Ocimum ciliatum L.) in two different harvesting times

نویسندگان [English]

  • Sara Farsari
  • Mohammad Moghaddam
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

In order to investigate the effect of mycorrhizal fungi and foliar application of putrescine on some biochemical characteristics, essential oil content, and biomass of basil (Ocimumciliatum L.), a split plot in time experiment with three replications was performed based on a completely randomized design. Treatments included three levels of mycorrhizal fungi (control, Glomusmossea, and Glomusintraradicese) and three levels of putrescine (0, 1, and 2 mM) as main factors and two harvesting times as sub-factor. Results indicated that application of mycorrhizal fungi and putrescine influenced  the biochemical traits, fresh and dry biomass of aerial parts, and essential oil content of the plants. The highest amount of chlorophyll a and essential oil content were observed at G. mossea and 2 mM putrescine application in the first harvesting time. The highest amounts of chlorophyll b and carotenoid were obtained at G. intraradicese and 2 mM putrescine application in the first harvesting time. But with increasing the day time length and temperature, the amount of photosynthetic pigments in the second harvesting time decreased. The highest antioxidant activity and total phenolic content were observed in the treatment of G.intraradicese without putrescine spraying in the second harvesting time. The highest fresh and dry biomass of aerial parts were observed by applying G. mossea; but, no significant difference was observed between the two concentrations of  putrescine and only a significant increase was recorded in comparison with no putrescine treatment. Generally, applying both types of mycorrhizal fungi as biofertilizers with foliar application of 2mM putrescine could significantly improve the traits in this study.

کلیدواژه‌ها [English]

  • antioxidant activity
  • biomass
  • Biofertilizer
  • Essential oil content
  • Photosynthetic pigments
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