بررسی خصوصیات فیزیولوژیک جلبک‌های سبز به منظور استفاده از آن‌ها به عنوان سوخت بیو‌دیزل

نوع مقاله: پژوهشی

نویسندگان

1 گروه زیست‌شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد علوم تحقیقات، تهران، ایران

2 گروه پژوهشی میکروبیولوژی نفت، پژوهشکده علوم پایه کاربردی جهاد دانشگاهی، تهران، ایران

3 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

چکیده

گرم شدن تدریجی زمین به‌دلیل استفاده بی‌رویه از سوخت‌های فسیلی، افزایش قیمت، آلودگی‌های زیست‌محیطی و تولید گازهای گلخانه‌ای سبب شده تا دانشمندان درصدد تولید سوختی تجدیدپذیر و جایگزین سوخت‌های فسیلی باشند. این مطالعه با هدف بررسی پتانسیل چهار گونه جلبک سبز به عنوان مواد خام تولید کننده چربی برای سنتز بیو‌دیزل انجام شد. بعد از خالص‌سازی، نمونه‌هادر محیط کشت BBM و N8 در دمای 25 درجه سانتی‌گراد تحت نور دائمی و 7 pH نگهداری شدند. نرخ رشد، میزان رنگیزه‌های کلروفیل، کاروتنوئیدها و میزان لیپید نمونه‌ها اندازه‌گیری شدند. میزان کل اسیدهای چرب با کمک دستگاه FTIR و پروفایل اسیدهای چرب با استفاده ازدستگاه GC-Mass سنجیده شد. نتایج نشان داد بالاترین میزان رشد و تولید بیومس و کمترین زمان تقسیم و همچنین بیشترین میزان تولید لیپید مربوط به جلبک سبزISC 94  Scenedesmus spمی‌باشد. در جلبک سبزISC 94  Scenedesmus sp، پالمتیک اسید (74/42 درصد)، استئاریک اسید (56/29 درصد)، پالمیتولئیک اسید (2/10 درصد)، اولئیک اسید (72/6 درصد)، لینولئیک اسید (72/1 درصد)، α- لینولنیک اسید (636/1 درصد) اندازه‌گیری شد. به علاوه، آنالیز لیپیدها نشان داد که 80 درصد اسیدهای چرب از نوع اشباع و غیراشباع با یک پیوند دوگانه بودند. همچنین پالمیتیک اسید و اولئیک اسید مهم‌ترین اسیدهای چرب جداسازی شده می‌باشند. بنابراینسندسموس به‌دلیل تولید بالاترین و بهترین محتوای لیپیدی می‌تواند به‌عنوان یک کاندید مناسب برای سوخت بیو‌دیزل معرفی گردد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Analysis of physiologic characteristics of green algae for biodiesel production

نویسندگان [English]

  • Ramazanali Khavarinejad 1
  • fatemeh malekahmadi 1
  • neda soltani 2
  • farzaneh najafi 3
  • Taher Nejadsattari 1
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Petroleum Microbiology, Research Institute of Applied Science, ACECR, Tehran, Iran
3 Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
چکیده [English]

Global warming, due to the excessive use of fossil fuels, rising prices, environmental pollution, and greenhouse gas emission, have made scientists produce a renewable fuel as a replacement for fossil fuels. The aim of this study was to evaluate the potential of four species of green algae as raw materials for biodiesel production. After purification, samples were kept in BBM and N8 culture medium at 25° C, pH 7, and under constant light. Growth rate, chlorophyll content, carotenoid concentration, and lipid content were measured. The total fatty acid contents and fatty acid profiles were measured with FTIR and GC-Mass, respectively. Findings showed that the highest growth rate and biomass production, and the minimum division time and also the maximum lipid contents belonged to the green algae Scenedesmussp ISC 94. Moreover, palmitic acid (42.74%), stearic acid (29.56%), palmitoleic acid (10.2%), oleic acid (6.72%), linoleic acid (1.72%), and α-linolenic acid (1.64%) were measured in Scenedesmussp ISC 94. The fatty acid composition of the microalgal lipid comprised over %80 of saturated and monounsaturated fatty acids with a double bound. Also, palmitic and oleic acids were the majore fatty acids isolated. Therefore, ecause of high lipid production and the best lipid content, Scenedesmussp ISC 94 is recommended for its potential as a biodiesel feedstock.
 

کلیدواژه‌ها [English]

  • Biodiesel
  • Fatty acid percent
  • Gc
  • Green algae
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