بررسی محتوی متابولیتی و فعالیت آنتی‌اکسیدانی اندام‌های مختلف علف‌هرز مهاجم نیلوفر پیچ (Ipomoea tricolor Cav.) در مزارع گنبد کاووس

نوع مقاله: پژوهشی

نویسندگان

1 گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران.

2 شناسایی و مبارزه با علف‌های‌هرز، دانشکده کشاورزی و منابع‌طبیعی دانشگاه گنبد کاووس، گنبدکاووس، ایران.

چکیده

هدف از این آزمایش، ارزیابی محتوی متابولیتی (اولیه و ثانویه) و فعالیت آنتی‌اکسیدانی اندام‌های مختلف علف‌هرز نیلوفر پیچ نظیر ساقه، برگ و میوه به‌علاوه مخلوطی از آن‌ها بود. ابتدا علف‌هرز مورد برسی در مرحله میوه‌دهی جمع‌آوری و به‌تفکیک اندام جدا گردید. فعالیت آنتی‌اکسیدانی اندام‌های مختلف نیلوفر پیچ به روش DPPH مورد اندازه‌گیری قرار گرفت. نتایج نشان داد که مخلوطی از اندام‌های علف‌هرز نیلوفر پیچ و سپس برگ از بیشترین مقدار فنل کل برخوردار بودند. در مورد آنتوسیانین‌ها، بیشترین مقدار آن‌ها به برگ اختصاص داشت. در حالی‌که ساقه از کمترین میزان هر دوی متابولیت‌های ثانویه برخوردار بود. هم‌چنین نتایج نشان داد که بیشترین میزان قندهای محلول و پرولین به اندام برگ اختصاص داشت. ضرایب همبستگی داده‌ها نشان داد که میزان آنتوسیانین‌ها در اندام‌های مختلف نیلوفر پیچ رابطه مثبت و معنی‌داری با قندهای محلول و اسید آمینه پرولین داشتند. مطابق نتایج، با افزایش میزان متابولیت‌ ثانویه آنتوسیانین‌ها در اندام‌های مختلف، میزان نشاسته و پروتئین به‌طور معنی‌داری کاهش یافت. مقایسه میانگین فعالیت آنتی‌اکسیدانی اندام‌های مختلف نیلوفر پیچ نشان داد که برگ و میوه به‌ترتیب از بیشترین و کمترین فعالیت آنتی‌اکسیدانی در مهار رادیکال‌های آزاد برخوردار بودند. با توجه به تاثیرگذاری نوع اندام علف ‌هرز نیلوفر پیچ بر کمیت محتوی متابولیتی به‌ویژه ترکیبات ثانویه نظیر فنل‌ها، آنتوسیانین‌ها به همراه فعالیت آنتی‌اکسیدانی، شاید بتوان این گیاه بخصوص اندام برگ را به‌عنوان کاندیدی قابل تامل برای تجزیه اکسیداتیو رادیکال‌های آزاد، بهبود ارزش تغذیه‌ای غذا و یا علف‌کش‌ها با منشاء طبیعی با توجه به زیست توده بالا معرفی نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of metabolites content and antioxidant activity of various organs of invasive weed of Ipomoea tricolor Cav. in Gonbad Kavous farms

نویسندگان [English]

  • Ebrahim Gholamalipour Alamdari 1
  • Alie Syedi 2
  • hossein sabouri 1
  • Zeinab Avarseji 1
  • Abbas Biabani 1
1 Plant Production Department, College of Agriculture and Natural Resources of Gonbad Kavous University, Gonbad Kavous, Iran.
2 Identification and Weeds Control Branch, Plant Production Department, College of Agriculture and Natural Resources of Gonbad Kavous University, Gonbad Kavous, Iran.
چکیده [English]

The purpose of this study was to evaluate metabolites content (primary and secondary) and antioxidant activity of different organs of Ipomoea tricolor weed including stem, leaf, and fruit leaf, individually and in combination. The weed under study was first collected at fruit stage and divided into organs. Antioxidant activities of various organs of Ipomoea tricolor were measured according to the method of DPPH. Results showed that mixed organs of Ipomoea tricolor and then leaf had maximum total phenol contents in that order. In case of anthocyanins, the highest levels were obtained from leaf while the lowest content of both secondary metabolites were found in the stem. Results also showed that leaves had maximum soluble sugars and proline contents. Correlation coefficient of data showed a significant positive relationship between anthocyanin contents of various organs of Ipomoea tricolor and their soluble sugar and proline contents. According to the findings, content of protein and starch in various organs significantly decreased with an increase in the secondary metabolite contents of anthocyanins. Mean comparison of antioxidant activity of various organs of Ipomoea tricolor showed that leaf and fruit had the highest and lowest antioxidant activities for inhibiting free radicals, respectively. Regarding the effect of the type of Ipomoea tricolor weed organ on the quantity of metabolites especially secondary compounds like phenols and anthocyanins and also antioxidant activity, the plant, especially the leaves may be introduced as a notable candidate for oxidative decomposition of free radicals, improving the nutritional value of food, or as a natural herbicide due to its high biomass.

کلیدواژه‌ها [English]

  • Anthocyanin
  • Leaf organ
  • DPPH method
  • Oxidative decomposition
  • Total phenol
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