اثر تیمار شوری بر رشد و برخی پارامترهای بیوشیمیایی گیاه سرخارگل ارغوانی (Echinacea purpurea L.)

نوع مقاله: پژوهشی

نویسندگان

گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

چکیده

سرخارگل (Echinaceae prupurea)، گیاهی علفی و چند ساله از تیره­ی کاسنی است که خواص دارویی متعددی از آن گزارش شده است. هدف از تحقیق حاضر بررسی اثر تنش شوری بر رشد و برخی پارامترهای بیوشیمیاییاین گیاه است. به این منظور گیاهچه­های سرخارگل به مدت 20 روز در محیط کشت هوگلند حاوی غلظت­های مختلف نمک (0، 25، 50، 75، 100 میلی­مولار) تیمار شدند. آزمایش در قالب طرح بلوک‌های تصادفی با 5 تکرار انجام شد.  نتایج حاصل نشان داد که شوری در سطوح مختلف اثر معنی­داری بر وزن خشک اندام هوایی نداشت. اما تیمار گیاهچه­ها با غلظت 25 میلی­مولار نمک سبب افزایش معنی­دار وزن خشک و تر ریشه گشت. همچنین تیمار شوری به‌ترتیب سبب افزایش و کاهش قندهای محلول بخش هوایی و ریشه شد. بررسی فعالیت آنزیم­های آنتی‌اکسیدانت نشان داد که شوری بر فعالیت آنزیم کاتالاز اثر معنی­داری نداشته و تنها در تیمار 75  میلی­مولار سبب افزایش معنی­دار فعالیت آنزیم پراکسیداز در هر دو بخش هوایی و ریشه شد. سنجش ترکیبات فنلی نیز نشان داد که تیمار شوری به‌ترتیب سبب کاهش و افزایش میزان این ترکیبات در بخش هوایی و ریشه گشت. نتایج حاصل از آنالیز HPLC میز نشان داد که بالاترین میزان شیکوریک اسید (3/1 میلی­گرم در هر گرم وزن خشک) مربوط به گیاهچه­های شاهد بود. در حالی که تیمار گیاهچه­ها با غلظت­های 25 و 50 میلی­مولار نمک اثر معنی­داری بر میزان شیکوریک اسید بخش هوایی نداست. از سویی دیگر تیمار 75 میلی­مولار نمک سبب کاهش میزان این ترکیب شد. نتایج تحقیق حاضر نشان داد که تیمار 50  میلی­مولار نمک به ترتیب سبب افزایش 2 و 5 برابری در میزان  کافئیک اسید (پیش ماده شیکوریک اسید) و کلروژنیک اسید در اندام ریشه شد. با توجه به شواهد حاضر به نظر می­رسد که با اعمال تنش شوری بتوان تولید ترکیبات با ارزش دارویی گیاه سرخارگل را افزایش داد.

کلیدواژه‌ها


عنوان مقاله [English]

The impact of salt stress on growth and some biochemical parameters of Echinaceae purpurea L.

نویسندگان [English]

  • Mahnaz Aghdasi
  • Mohammad Fatemi
  • Asieh Asadi
Deptartment of Biology, Faculty of Science, Golestan University, Gorgan, Iran
چکیده [English]

Echinaceae prupurea, belongs to the Asteraceae family and is a perennial herb with different medicinal properties. The aim of the present study was to investigate the effect of salt stress on growth and some important biochemical parameters of this plant. For this purpose, Echinaceae prupurea plantlets were grown in Hoagland medium supplemented with different concentrations of NaCl (including 10, 50, 100, 300, and 500 mM) for 20 days. The experiment was carried out in a completely randomized design with five replications. The obtained result showed that the effects of different concentrations of NaCl were not significant on shoot dry weights. But root fresh and dry weights exhibited a significant increasing trend by 25 mM NaCl treatment. Meanwhile, the current data showed that increasing salt concentration in the medium caused an increase and decrease in soluble sugar level in shoots and roots, respectively. While salt stress did not show any significant effect on catalase enzyme activity, peroxidase enzyme activity was significantly increased by 75 mM NaCl treatment, in both shoots and roots. On the other hands, salinity treatment significantly decreased and increased total phenol levels in shoots and roots, respectively. The data from HPLC analysis demonstrated that the highest level of cichoric acid (1.3 mg/g dry weight) was observed in roots of control samples. While NaCl at the level of 25 and 50 mM concentrations did not show any significant effect on cichoric acid amount, it was significantly decreased by 75 mMN aCl treatment. Moreover, 50 mM NaCl treatment enhanced 2 and 5 fold caffeic acid (precursor of cichoric acid) and chlorogenic acid level in root organ, respectively. It seems salt stress can increase the important medicinal secondary metabolites of Echinaceae purpurea.

کلیدواژه‌ها [English]

  • Caffeic acid
  • Chlorogenic acid
  • Cichoric acid
  • Echinaceae prupurea
  • growth
  • phenol
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