تنوع فیتوشیمیایی، بیوشیمایی و مولکولی انجیر (Ficus carica L.) در استان آذربایجان شرقی

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه آتاترک، ارزروم، ترکیه

چکیده

انجیر (Ficus carica) درخت بومی مناطق غرب و شرق مدیترانه از جمله ایران می­باشد. ایران یکی از کشورهای مهم تولید کننده انجیر در جهان می­باشد. در این  پژوهش تنوع صفات بیوشیمایی و فیتوشیمیایی میوه و تنوع مولکولی 38 ژنوتیپ انجیر منطقه ارسباران استان آذربایجان شرقی در سال 1396 و در آزمایشگاه علوم باغبانی دانشگاه ارومیه مورد بررسی قرار گرفتند. با توجه به نتایج تجزیه واریانس صفات اندازه­گیری شده از قبیل مواد جامد محلول، pH، شاخص طعم، ویتامین ث، ظرفیت آنتی­اکسیدانی، آنتوسیانین، فلاونوئید و فنل­ کل میوه در سطح احتمال یک درصد معنی­دار بودند. بطوریکه در بیشتر صفات بیوشیمیایی مورد بررسی تنوع بسیار بالایی مشاهده گردید. همچنین نتایج داده­های مولکولی نیز نشان داد در مجموع 131 باند تکثیر شده بود که از این میزان 121 باند (92 درصد) چند شکل بودند. میزان PIC برای آغازگرهای مورد استفاده در تجزیه ISSR بین 22/0 در آغازگر ISSR7 تا 47/0 در آغازگر ISSR20 متغیر بود. همچنین شاخص نشانگری از 51/0 تا 50/2 برای نشانگرهای ISSR7 و ISSR20 متفاوت بود. بر اساس نتایج تجزیه خوشه­ای، ژنوتیپ­های مورد بررسی انجیر در دو گروه اصلی قرار گرفتند که اکثر ژنوتیپ­های جمع آوری شده از کلیبر در گروه دوم قرار داشتند که نشان دهنده این است که موقعیت جغرافیایی ژنوتیپ­های مورد بررسی انجیر عامل اصلی تفکیک آنها به وسیله این نشانگر می­باشد. با توجه به نتایج حاصله می­توان ژنوتیپ­های Kh9 و H17 را برای برنامه­های اصلاحی آینده پیشنهاد نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Variation on biochemical, phytochemical and genetic diversity of fig (ficus carica) from East Azerbaijan province

نویسندگان [English]

  • akbar ghorbani 1
  • hamid Hasanpour 1
  • sezai Ercisli 2
1 Department of Horticulture, Faculty of Agriculture, Urmia University
2 Department of Horticulture, Faculty of Agriculture, Ataturk University, Turkey
چکیده [English]

Abstract. Fig (Ficus cariaca) is native to western and eastern mediterranean regions such as western and northwestern of Iran. Iran is one of the important fig producers in the world. In this study, biochemical, phycochemistry characteristics and genetic diversity of 38 fig genotypes from Arsbaran in East Azerbaijan province in the Horticulture Laboratory of Urmia University in 2015 were investigated. Biochemical results showed that, TSS, pH, TSS/TA, vitamin c, antioxidant capacity, total anthocyanin, total flavonoid and total phenol were significant (p ≤ 0.01). Most of the studied biochemical traits indicated high variation. The results of the molecular data showed that a total of 131 bands were scored, of which 121 bands (92%) were polymorphic. The amount of polymorphic (PIC) information for the primers used varied from 0.22 to 0.47 in ISSR7 and ISSR20 respectively. Also, marker index were between 0.15 to 2.5 in ISSR7 and ISSR20 respectively. Based on the results of cluster analysis, the studied fig genotypes were classified into two main groups, so that the most of collected genotypes from Kalibar belonged to second group. So it could be concluded that geographic location of the studied fig genotypes is the main reason for their separation by this marker. Based on obtained results, the genotypes Kh9 and H17 were suggested for breeding programs.

کلیدواژه‌ها [English]

  • Anthocyanin
  • Antioxidant capacity
  • Cluster analysis
  • ISSR markers
  • vitamin C
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