تعیین مهم‌ترین صفات مؤثر بر نشت الکترولیتی گلابی وحشی (Pyrus biossieriana Buhse) تحت شرایط تنش خشکی با استفاده از روش‌های آماری چند متغیره

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

3 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

گلابی وحشی یک پایه مهم برای ارقام مختلف گلابی محسوب می‌شود و با توجه به اینکه تا حدودی بردبار به خشکی بوده، کاربرد تیمارهای مختلف برای افزایش تحمل آن در مقابله با تنش خشکی امری ضروری است. نشت الکترولیت‌ها عامل مهم و مؤثر در پاسخ به تنش خشکی و صفات مربوط به آن می‌باشد. برای این منظور آزمایش فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار جهت شناسایی صفات مؤثر بر نشت الکترولیتی طراحی گردید. این صفات در 12 تیمار شامل سطوح مختلف تنش خشکی و غلظت‌های مختلف براسینواستروئید روی گلابی وحشی در بهار و تابستان سال 1395 در شرایط گلخانه انجام شد. نتایج همبستگی سایر صفات با نشت الکترولیت نشان داد که این صفت با صفات میزان پرولین (**83/0r= )، مالون دآلدئید (**98/0r= ) و پراکسید هیدروژن (**87/0r= ) همبستگی مثبت و کاملاً معنی‌دار و با صفات وزن خشک ریشه، برگ و ساقه، رشد طولی، کلروفیل a، b و کاروتنوئید همبستگی منفی و کاملاً معنی‌دار داشت. نتایج رگرسیون گام به گام نشان داد که چهار صفت کلروفیل a، وزن خشک برگ، رشد طولی و پراکسید هیدروژن در مجموع 99% از تغییرات را توجیه نمودند. بر اساس نتایج تجزیه علیت، میزان پراکسید هیدروژن بیشترین تأثیر مستقیم مثبت (083/0) و صفات رشد طولی و کلروفیل a بیشترین تأثیر مستقیم و منفی را بر نشت الکترولیت‌ها داشتند. صفت میزان پراکسید هیدروژن بیشترین اثر غیرمستقیم و مثبت را از طریق کاهش میزان کلروفیل a بر نشت الکترولیت‌ها داشت. صفت رشد طولی بیشترین اثر غیرمستقیم و منفی را از طریق کلروفیل a بر نشت الکترولیت‌ها داشت. با استفاده از نتایج این پژوهش می‌توان علاوه بر اندازه‌گیری نشت الکترولیت‌ها به صورت مستقیم، تیمارهای افزایش تحمل به تنش خشکی در گلابی وحشی را با اندازه‌گیری میزان پراکسید هیدروژن، رشد طولی و کلروفیل a توصیه نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Determination of the most important traits affecting the electrolyte leakage of wild pear (Pyrus biossieriana Buhse) under drought stress conditions using multivariate statistical methods

نویسندگان [English]

  • seyed morteza zahedi 1
  • Mahdieh Karimi 2
  • Farzad Kian Ersi 3
1 Department of Horticultural Science, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
2 Department of Horticulture Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
3 Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
چکیده [English]

Wild pears are an important rootstock for different pear cultivars and considering it is nearly tolerant to drought stress it is necessary to use different treatments to increase its tolerance to drought stress. Electrolytes leakage is an important and effective factor in response to drought stress and related traits. For this purpose, a factorial experiment was designed in a randomized complete blocks design with three replications to identify the traits affecting electrolytes leakage. These traits were studied in 12 treatments including different levels of drought stress and different concentrations of brassinosteroids on wild pears in spring and summer of 2017 in greenhouse conditions. The result showed a positive significant correlation between proline content (0.83**), malondialdehyde (0.98**), hydrogen peroxide and electrolyte leakage. The correlation for dry weight of root, leaf and stem, height growth, chlorophyll a, b and carotenoid was significantly negative. Stepwise regression analysis indicated that chlorophyll a, leaf dry weight, height growth and hydrogen peroxide were the most important effective traits on electrolytes leakage and showed 99% of total variance. Based on the path analysis results, the traits of electrolytes leakage and height growth and chlorophyll a showed the maximum positive direct and significant at p

کلیدواژه‌ها [English]

  • Correlation
  • Electrolytes leakage
  • path analysis
  • Stepwise regression
  • Wild pears
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