بررسی خصوصیات فیزیولوژیکی و فعالیت آنتی‌اکسیدانی ریحان (Ocimum basilicum cv. Keshkeni luvelou)تحت سطوح مختلف متیل‌جاسمونات و سمیت سرب

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهدف مشهد، ایران.

3 مرکز تحقیقات گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهرقدس، تهران، ایران

4 گروه علوم خاک، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

آزمایشی به منظور بررسی تاثیر متیل­جاسمونات بر خصوصیات فیزیولوژیکی و فعالیت آنتی­اکسیدانی گیاه ریحان تحت سمیت سرب بصورت فاکتوریل در قالب طرح کاملا ً تصادفی در سه تکرار در گلخانه تحقیقاتی دانشگاه فردوسی مشهد انجام شد. تیمارهای آزمایش شامل سه سطح نیترات سرب (صفر (شاهد)، 200 و 400 میلی­گرم در کیلوگرم خاک) و محلول­پاشی متیل­جاسمونات در سه غلظت (صفر، 5/0 و 1 میلی‌مولار) بودند. صفات مورد ارزیابی شامل میزان کلروفیل a و b، کلروفیل کل، کارتنوئیدها، نشت الکترولیت، رطوبت نسبی، فعالیت آنتی‌اکسیدانی، کربوهیدرات­های محلول، فنل کل و پرولین بود. نتایح حاصل از این مطالعه نشان داد که در گیاهان تیمار شده با سرب، میزان پرولین، محتوای نسبی آب، کلروفیلa ، فعالیت آنتی­اکسیدانی، فنل کل در مقایسه با گیاهان شاهد کاهش معنی­داری نشان داد و کاربرد متیل‌جاسمونات (5/0 میلی­مولار) در شرایط تنش سرب، باعث افزایش این صفات گردید. علاوه بر این در شرایط تنش سرب، میزان نشت الکترولیت به میزان قابل توجهی افزایش یافت و محلول­پاشی متیل­جاسمونات سبب کاهش آن شد. بنابراین، استفاده از متیل‌‌جاسمونات برای حفظ عملکرد اقتصادی گیاهان تحت تنش قابل توجیه است. همچنین استفاده از غلظت‌های 5/0 و 1 میلی­مولار آن در شرایط تنش عنصر سنگین سرب با تحریک ساخت آنتی اکسیدانت­ها، منجر به کاهش و تعدیل اثرات این تنش می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of physiological characteristics and antioxidant activity of sweet basil (Ocimum basilicum cv. Keshkeni luvelou) under different levels of methyl jasmonate and lead toxicity

نویسندگان [English]

  • Somaye Asadi 1
  • Mohammad Moghaddam 2
  • Abdollah Ghasemi Pirbalouti 3
  • Amir Fotovat 4
1 Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Research Center for Medicinal Plants, Shahr-e-Qods Branch, Islamic Azad University, Shahr-e-Qods, Tehran, Iran.
4 Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

This study was performed in order to evaluate the effect of methyl jasmonate on some physiological characteristics and antioxidant activity of basil under lead toxicity, based on a factorial and completely randomized design with three replications at Ferdowsi University of Mashhad Research Greenhouse. The experimental treatments included Pb (NO3)2) at three levels of (0, 200, and 400 mg/kg soil) and foliar application of methyl jasmonate at three concentrations (0, 0.5, and 1 mM). Measured traits included chlorophyll a, b, and total, carotenoids, electrolyte leakage, relative water content, antioxidant activity, soluble carbohydrate, total phenol, and proline. Results showed that in the plants treated with lead, the amount of proline, relative water content, chlorophyll a, antioxidant activity and total phenol reduced in comparison with control plants and application of methyl jasmonate (0.5 mM) under lead stress increased these traits. Furthermore, electrolyte leakage increased significantly under lead stress, while it was decreased by spraying with methyl jasmonate. Therefore, using methyl jasmonate is justified for protecting economic performance of plants under stress. Also, application of 0.5 and 1 mM methyl jasmonate reduced and adjusted the effects of stress in the plants under lead stress through stimulating production of antioxidants.

کلیدواژه‌ها [English]

  • Electrolyte leakage
  • Growth regulator
  • Photosynthetic pigments
  • Proline
  • Relative water content
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