بررسی پاسخ فیزیولوژیک و دفاع آنتی‌اکسیداتیو خردل سفید (Sinapis alba) تحت تنش کادمیم و سرب

نوع مقاله: پژوهشی

نویسندگان

گروه زیست شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران

چکیده

فلزات سنگین از مهم‌ترین آلاینده‌های خاک و محیط زیست می‌باشند. در این تحقیق، برخی پارامترهای بیوشیمیایی گیاه  Sinapis alba  رشد یافتهدر خاک‌های‌ آلوده شده با غلظت‌های مختلف کادمیم (۰، 75 و 150 mg/kg) و سرب (۰، 300 و 600 mg/kg) مورد بررسی قرار گرفت. گیاهان حدود ۷ هفته پس از کشت در گلخانه‌، برداشت شدند. نتایج مطالعه نشان داد که تیمارهای کادمیم سبب کاهش طول و وزن تر بخش هوایی و کل گیاه شد. هر چند تیمارهای سرب اثر معنی‌داری در صفات رشد گیاه نداشت. تیمارهای کادمیم سبب کاهش فعالیت آنزیم کاتالاز، میزان پراکسید هیدروژن، پراکسیداسیون لیپید و افزایش میزان پروتئین محلول در بخش هوایی و افزایش فعالیت آنزیم پلی فنل اکسیداز در بخش هوایی و ریشه گیاه در مقایسه با شاهد شد. گیاهان تیمار شده با ۷۵ mg/kg کادمیم در مقایسه با گیاهان شاهد میزان کلروفیل a، کلروفیل کل و نسبت کلروفیل a به b بیشتری را نشان دادند. تیمارهای سرب منجر به کاهش، فعالیت آنزیم آسکوربات پراکسیداز در بخش هوایی و ریشه، میزان پروتئین محلول در ریشه و قندهای غیراحیاء کننده در بخش هوایی شد. تیمار 300 mg/kg سرب در بخش هوایی سبب افزایش، فعالیت آنزیم گایاکول پراکسیداز محلول و دیواره‌ای، میزان پراکسید هیدروژن، پروتئین محلول و کلروفیل a، b و کل گردید. تیمار ۶۰۰ mg/kg سرب سبب افزایش میزان پراکسید هیدروژن ریشه و کاهش کاروتنوئیدها شد. این نتایج نشان می‌دهد که خردل سفید با توجه به بیوماس بالا، افزایش میزان رنگدانه‌های فتوسنتزی و عدم وجود تنش اکسیداتیو دارای توانایی قوی برای تحمل کادمیم و سرب می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of salicylic acid on some growth and biochemical characters of Solanum tuberosum cv. Agria under salinity stress on in vitro condition

نویسندگان [English]

  • Ahmad Abdolzade
  • Zahra Soleiman nejad
  • Hamid reza Sadeghe pour
Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
چکیده [English]

Salinity is an abiotic stress that seriously constrains agricultural production including potatoes in most regions of the world. This study was carried out to investigate the effect of salicylic acid, on growth properties and biochemical characteristics of in vitro cultureof Solanum tuberosum cv. Agria under salinity stress. The study was carried out at a completely random design with eight replications in the Department of Horticulture of University of Tabriz. Variables under study included salinity stress at two levels (0 and 70 mM/L sodium chloride) and salicylic acid at four levels (0, 1, 10, and 100 mM/L). Results showed that salicylic acid significantly mitigated the effects of salinity. Therefore, the highest plantlet length was recorded under salinity along with 10 mM salicylic acid treatment showing the positive effect of the treatment on mitigating the influences of salinity. However, the concentrations of salicylic acid higher than 10mM/L not only had no effect on plants, but also negatively influenced the effects of salinity stress. Also, antioxidant enzyme activity of the plantlets had a considerable increase at all concentrations of salicylic acid and levels of salinity. Moreover, the study suggested that Solanum tuberosum cv. Agria was relatively sensitive to salinity stress and all characteristics of the plants under study were influenced with salinity. Also, application of salicylic acid caused improvement in the growth and biochemical properties of the plants under study increasing their tolerance against salinity stress.
 

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • In vitro
  • Morphological Characteristics
  • Salicylic Acid
  • Salinity
  • Solanum tuberosum L
  • Tissue culture
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