مطالعه درون شیشه‌ای اثر اسید سالیسیلیک بر برخی ویژگی‌های رشدی و بیوشیمیایی سیب‌زمینی رقم آگریا (Solanum tuberosum cv. Agria )تحت تنش شوری

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

2 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

3 گروه علوم و مهندسی باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران.

چکیده

تنش شوری، یک تنش محیطی است که رشد و نمو گیاهان و تولید محصولات کشاورزی از جمله سیب زمینی را در بیشتر نقاط جهان متاثر می‌سازد. این پژوهش با هدف بررسی اثر سالیسیلیک اسید بر صفات رشدی و بیوشیمیایی سیب زمینی رقم آگریا تحت تنش شوری در شرایط درون شیشه‌ای انجام شد. برای این منظور، آزمایشی در قالب طرح‌های کاملاً تصادفی با 8 تکرار در گروه باغبانی دانشگاه تبریز به اجرا درآمد. عامل‌های آزمایش، شامل شوری در دو سطح (صفر و 70 میلی‌مول بر لیتر کلرید سدیم)، اسید سالیسیلیک در چهار سطح (صفر، 1، 10 و 100 میلی‌مول بر لیتر) بود. نتایج نشان داد که استفاده از اسید سالیسیلیک توانسته است به‌طور معنی‌داری اثرات شوری را کاهش دهد، با این وجود بالاترین طول گیاهچه در تیمار شوری در 10 میلی­مول بر لیتر اسید سالیسیلیک مشاهده شد که حاکی از اثرات مثبت تیمار در کاهش اثرات منفی تنش شوری است، با این‌حال غلظت‌های بالاتر از 10 میلی­مول بر لیتر نه تنها تاثیری در گیاهچه­ها نداشته، حتی باعث اثرات منفی شدیدی نیز گردید. همچنین فعالیت ترکیبات و آنزیم­های آنتی‌اکسیدان در تمام غلظت­های بررسی شده اسید سالیسیلیک و تنش شوری نسبت به گیاه شاهد افزایش قابل توجهی نشان داد. بررسی نتایج حاصل از آزمایش نشان داد که سیب‌زمینی رقم آگریا نسبتاً به شوری حساس بوده، به گونه‌ای که کلیه صفات مورد بررسی در آزمایش تحت تأثیر اولین سطح شوری اعمال شده قرار گرفتند. همچنین کاربرد اسید سالیسیلیک با کمک به بهبود ویژگی­های رشدی و بیوشیمیایی موجب افزایش تحمل این رقم در برابر تنش شوری گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of salicylic acid on some growth and biochemical characters of Solanum tuberosum cv. Agria under salinity stress on in vitro condition

نویسندگان [English]

  • farzaneh Fakhimi 1
  • Alireza Motallebi Azar 1
  • Fariborz Zaree Nahandi 1
  • Nemat Sokhandan Bashir 2
  • Gholamreza Gohari 3
1 Department of Horticulture, Faculty Agriculture, University of Tabriz, Tabriz, Iran.
2 Department of Plant Protection, Faculty Agriculture, University of Tabriz, Tabriz, Iran
3 Department of Horticulture, Faculty Agriculture, University of Maragheh, Maragheh, Iran.
چکیده [English]

Salinity is an abiotic stress that seriously constrains agricultural production including potatoes in most regions of the world. This study was carried out to investigate the effect of salicylic acid, on growth properties and biochemical characteristics of in vitro cultureof Solanum tuberosum cv. Agria under salinity stress. The study was carried out at a completely random design with eight replications in the Department of Horticulture of University of Tabriz. Variables under study included salinity stress at two levels (0 and 70 mM/L sodium chloride) and salicylic acid at four levels (0, 1, 10, and 100 mM/L). Results showed that salicylic acid significantly mitigated the effects of salinity. Therefore, the highest plantlet length was recorded under salinity along with 10 mM salicylic acid treatment showing the positive effect of the treatment on mitigating the influences of salinity. However, the concentrations of salicylic acid higher than 10mM/L not only had no effect on plants, but also negatively influenced the effects of salinity stress. Also, antioxidant enzyme activity of the plantlets had a considerable increase at all concentrations of salicylic acid and levels of salinity. Moreover, the study suggested that Solanum tuberosum cv. Agria was relatively sensitive to salinity stress and all characteristics of the plants under study were influenced with salinity. Also, application of salicylic acid caused improvement in the growth and biochemical properties of the plants under study increasing their tolerance against salinity stress.

کلیدواژه‌ها [English]

  • Antioxidant enzymes
  • In vitro
  • Morphological Characteristics
  • Salicylic Acid
  • Salinity
  • Solanum tuberosum L
  • Tissue culture
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