تعیین کلون‌های متحمل به تنش خشکی چای (Camellia sinensis L.) با بررسی فعالیت آنزیم‌های آنتیاکسیدانت

نوع مقاله: پژوهشی

نویسندگان

1 گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران.

2 گروه بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران.

3 پژوهشکده چای، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، لاهیجان، ایران.

چکیده

به‌منظور بررسی فعالیت آنزیم‌های آنتی‌اکسیدانت 14 کلون چای در شرایط نرمال و تنش خشکی، دو آزمایش جداگانه به‌صورت طرح بلوک‌های کامل تصادفی با دو تکرار در سال زراعی 1396 در ایستگاه تحقیقات چای فشالم (رشت) اجرا گردید. در هر دو طرح، آبیاری به‌صورت معمول تا آخر تیر ماه انجام شد؛ اما در تیمار خشکی، از ابتدای مرداد تا موقع برداشت برگ‌های چای، آبیاری قطع گردید. در آخر مرداد، از هر کرت در هر دو آزمایش، برگ کلون‌های چای برداشته شده و به فریزر 80- منتقل شدند. فعالیت آنزیم‌های آنتی‌اکسیدانت آسکوربات پراکسیداز، سوپراُکسیددیس‌موتاز، کاتالاز، فنیل‌آلانین آمونیالیاز، لیپید پراکسیداز، میزان مالون‌دی‌آلدئید، بتاکاروتن و لیکوپن اندازه‌گیری شدند. نتایج نشان داد که تنش خشکی باعث افزایش فعالیت آنزیم‌های آنتی‌اکسیدانت گردید. بر اساس نتایج به‌دست‌آمده از مقایسات میانگین، کلون‌های 100، 399 و بذری تحت شرایط تنش خشکی دارای بیشترین مقادیر برای بیشترین مقدار فعالیت آنزیم‌های آنتی‌اکسیدانت آسکوربات پراکسیداز، سوپراُکسیددیس‌موتاز، کاتالاز، پراکسیداز، فنیل‌آلانین آمونیالیاز، لیپید پراکسیداز بودند. در طرف مقابل، این کلو‌ن‌ها کمترین مقدار را برای مالون‌دی‌آلدئید دارا بودند. این مساله تحمل نسبی آن‌ها را به خشکی نشان می‌دهد. کلون‌های 278 و 276 با کمترین مقدار آنزیم‌های آنتی اکسیدانت در شرایط تنش خشکی (بجز مالون‌دی‌آلدئید) به‌عنوان کلون‌های حساس در نظر گرفته شدند.

کلیدواژه‌ها


عنوان مقاله [English]

Determination of drought tolerance clones in Tea (Camellia sinensis L.) by investigating the activity of antioxidant enzymes

نویسندگان [English]

  • Mehdi Rahimi 1
  • Mojtaba Kordrostami 2
  • Mojtaba Mortezavi 1
  • Sanam Safaei-Chaeikar 3
1 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
2 Department of Plant Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
3 Tea Institute , Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran
چکیده [English]

In order to investigate the activity of antioxidant enzymes of 14 tea clones under normal and drought stress conditions, two separate experiments were carried out in a randomized complete block design with two replications in 2017 at Fashalem Tea Research Station in Rasht. Irrigation in both designs was carried out routinely until July 22 followed by a drought stress treatment for one plot until August 22 when tea leaves were harvested. Tea leaves from each plot of the both experiments were then removed and transferred to a freezer at -80 °C. The activity of the antioxidant such as ascorbate peroxidase, superoxide dismutase, catalase, peroxidase, phenylalanine ammoniaase, lipid peroxidase, malondialdehyde, β-carotene, and lycopene were measured. The results showed that drought stress increased the activity of antioxidant enzymes. Based on the results obtained from the mean comparisons, clones 100, 399 and Bazri, had the highest activity for ascorbate peroxidase, superoxide dismutase, catalase, peroxidase, phenylalanine ammoniaase, and lipid peroxidase under drought stress conditions. On the other hand, these clones had the lowest contents of malondialdehyde. Clones 278 and 276 on the other hand, had the least values of antioxidant enzymes under drought stress conditions (except malondialdehyde) and were considered as sensitive clones.

کلیدواژه‌ها [English]

  • : β-carotene
  • Catalase
  • Drought tolerance
  • Malondialdehyde
  • Peroxidase
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