ارزیابی ژنتیکی برخی جمعیت‌های گیاه دارویی زیره‌ سیاه (Carum carvi)با استفاده از نشانگرهای RAPD و ISSR

نوع مقاله: پژوهشی

نویسندگان

1 گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

2 روه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

3 پژوهشکده کشاورزی دانشگاه زابل، زابل، ایران

چکیده

در این تحقیق به‌منظور، تعیین رابطه خویشاوندی و فاصله ژنتیکی بین جمعیت‌های زیره­ی سیاه از نشانگرهای RAPD و ISSR استفاده شد. با استفاده از نشانگر RAPD در مجموع 126 آلل تکثیر شد که آغازگر TIBMBC08 و TIBMBA02 با 7 آلل کمترین تعداد و آغازگر  TIBMBC05با 22 آلل بیشترین تعداد داشتند. در نشانگر ISSR در مجموع 79 الل شناسایی که آغازگر UBC112 با 1 الل کمترین تعداد و آغازگر UBC809 با 15 آلل بیشترین تعداد داشتند. در نشانگر RAPD، بیشترین میزان شاخص چند شکلی با میزان 94/0 مربوط به آغازگر  TIBMBC05و کمترین میزان شاخص چندشکلی با میزان 8/0 مربوط به آغازگر TIBMBA02 و میزان شاخص تنوع شانن (57/0) و شاخص تنوع نی (38/0) مشاهده گردید. همچنین در نشانگر ISSR، بیشترین میزان شاخص چندشکلی با میزان 92/0 مربوط به آغازگرهای UBC809 و کمترین میزان شاخص چندشکلی مربوط به آغازگر  UBC112با میزان صفر و میزان شاخص تنوع شانن (57/0) و شاخص تنوع نی (39/0) مشاهده گردید. بر اساس اطلاعات RAPD و ISSR به‌صورت توأم بیشترین میزان تشابه مربوط به زیره­ی سیاه کرمان و زیره­ی سیاه جندق (48/0) و کمترین میزان تشابه مربوط به ژنوتیپ­های زیره­ی سیاه کرمان و زیره­ی سیاه نیشابور (115/0) با میانگین 315/0 می­باشد. تجزیه خوشه‌ای جمعیت‌های زیره­ی سیاه را بر اساس اطلاعات توأم هر دو نشانگر RAPD و ISSR در سه گروه مختلف که تا حدودی با موقعیت جغرافیایی آنها مطابفت داشت، طبقه بندی و از همدیگر تفکیک نمود.
 

کلیدواژه‌ها


عنوان مقاله [English]

Genetic assessment of some populations of the medicinal plant Caraway (Carum carvi) using RAPD and ISSR markers

نویسندگان [English]

  • leila fahmideh 1
  • Lila Jani pour 2
  • Bahman Fazeli-Nasab 3
1 Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran
2 Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran
3 Agricultural Research Center of Zabol University, Zabol, Iran
چکیده [English]

RAPD and ISSR markers were used in the present study to determine genetic relationship and distance between some populations of Carum carvi. Amplification of genomic DNA all population using RAPD analysis yielded 126 fragments, where TIBMBA02 and TIBMBA08 had the minimum number of fragments (7) and  TIBMBC05 had the maximum number of fragments, (22). ISSR marker yielded 79 fragments, where UBC112 and UBC809had the minimum (1) and maximum (15) fragments, respectively. The Diversity Index value of RAPD primers ranged from 0.8 (TIBMBA02) to 0.94 (TIBMBC05) and also Shanoon Diversity and Nei diversity were 0.57 and 0.38, respectively. In ISSR primers The Diversity Index value ranged from 0.0 (UBC112) to 0.92 (UBC809) and also Shanoon Diversity and Nei diversity were 0.57 and 0.39 respectively. According to ISSR and RAPD markers in combination, maximum genetic similarity (0.48) was observed between Kerman and Jandagh Carum carvi while the minimum similarity (0.115) was observed between Kerman and Neishabour Carum carvi with average 0.315.  Also, cluster analysis segregated all populations into 3 groups according to geographical zone.

کلیدواژه‌ها [English]

  • genetic diversity
  • Cluster analysis
  • Carum carvi
  • Diversity Index
  • RAPD and ISSR markers
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