بررسی عملکرد دانه، میزان کلروفیل، مواد اسمولیتی، فنول کل و فعالیت آنزیم کاتالاز ذرت (Zea mays L.) در پاسخ به تنش خشکی

نوع مقاله: پژوهشی

نویسندگان

1 گروه علوم کشاورزی، دانشگاه پیام نور، تهران، ایران

2 گروه زراعت واصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

به‌منظور بررسی عملکرد دانه، میزان کلروفیل (a+b)، گلایسین بتائین، پرولین، قندهای محلول، پلی‌فنول کل و فعالیت آنزیم کاتالاز در هیبریدهای ذرت در پاسخ به تنش خشکی، آزمایشی به‌صورت کرت­های خردشده در قالب طرح بلوک‌های کامل تصادفی در چهار تکرار طی دو سال زراعی 1390 و 1391 در مزرعه ایستگاه تحقیقاتی مغان اجرا شد. عامل اصلی تنش خشکی در دو سطح (آبیاری عادی و قطع آبیاری در مرحله پر شدن دانه) و عامل فرعی هیبریدهای ذرت (NS640، SC704 و SC720) بودند. تجزیه مرکب داده­ها نشان داد که تنش خشکی بر همه صفات اثر معنی­داری داشت. تنش خشکی باعث کاهش عملکرد دانه و میزان کلروفیل (a+b) شد. اما تنش خشکی باعث افزایش میزان گلایسین بتائین، پرولین، قندهای محلول، پلی فنول کل و فعالیت کاتالاز در برگ­های هیبرید ذرت مورد مطالعه گشت. سینگل کراس 704 دارای بیشترین عملکرد دانه و مواد اسمولیتی را در بین هیبریدهای ذرت مورد مطالعه به خود اختصاص داد. براساس نتایج حاصل، می­توان از عملکرد دانه، میزان کلروفیل، مواد اسمولیتی (گلایسین بتائین و پرولین)، قندهای محلول، پلی فنول کل و فعالیت آنزیم کاتالاز برای بررسی نحوه پاسخ هیبریدهای ذرت به تنش خشکی و شناسایی ارقام متحمل استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Grain yield, chlorophyll content, osmolyte accumulation, total phenolics and catalase activity in maize (Zea mays L.) under drought stress

نویسندگان [English]

  • Vahid Nasrollahzade asl 1
  • Sajjad Moharramnejad 2
  • Mehri Yusefi 1
1 Department of Agricultural Sciences, Payam-e-Noor University, Tehran, Iran
2 Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

To study the response of grain yield, chlorophyll (a+b) content, glycine betaine, proline, soluble sugars, total phenolics, and catalase (CAT) activity in maize hybrids to drought stress during kernel filling stage, a field experiment was carried out as a split plot completely randomized block design with four replications in Moghan Research Station, during 2010 and 2011 growing seasons. The main plots were composed of two drought stress levels including commonly available irrigation water (control) and no irrigation at kernel filling stage. Three maize hybrids including NS640, SC704, and SC720 were allocated into sub-plots. Analysis of the data suggested that drought stress significantly affected all measured characters and reduced grain yield  and chlorophyll (a+b) content. Drought stress also increased glycine betaine, proline, soluble sugars, total phenolics, and CAT activity about 30.2, 36.7, 17.8, 38.8 and %15.2, respectively. SC704 displayed maximum grain yield and osmolyte accumulation. Based on the findings of the study, grain yield, chlorophyll (a+b) content, glycine betaine, proline, soluble sugars, total phenolics, and catalase (CAT) activity can be used to study drought-response and drought-tolerance of maize hybrids.
 

کلیدواژه‌ها [English]

  • Proline
  • Drought stress
  • maize
  • Catalase activity
  • Soluble sugars
  • Grain yield
 

 

 

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