عنوان مقاله [English]
In order to investigate the effect of foliar application of methanol and drought stress on some biochemical properties of soybean (Glaycine max L.), a pot experiment was done in farm environment. The experiments were done in factorial form based on a completely random design with 3 repetitions. Treatments included three irrigation levels of normal (irrigation after 40% depletion of available soil moisture), average stress (irrigation after 60% depletion of available soil moisture), and severe stress (irrigation after 70% depletion of available soil moisture) as the main factor and levels of methanol in the form of foliar application including control solutions (foliar application without use of methanol) and solutions of 14.7% and 21% of methanol as secondary factor. Results obtained from the study showed that there were significant differences between various levels of methanol in content of chlorophyll and carotenoid compounds, relative water content, phenolic compounds, total protein content, proline, and leaf peroxide hydrogen in (P≤0.01). With the application of stress from mild to severe, application of 14% methanol showed more pronounced effects on total chlorophyll content, chlorophyll a and chlorophyll b. Moreover, under mild and severe stress conditions, with application of the highest value of methanol, production of peroxide hydrogen reached lowest level and the content of phenolic compounds increased with the increased application of methanol from 7% to 14%. According to the obtained results, with application of 14% methanol, more increase was observed in the efficiency of proline under severe stress conditions. Increasing the volume of methanol from 7 to 14%, the relative water content was preserved under stress conditions. For protein, with increased drought stress, the effect of application of 14% methanol was the same in comparison with 21% methanol. Therefore, according to the obtained results, it is concluded that methanol could improve plant resistance against drought stress.
Ahmed, S., Nawata, E., Hosokawa, M., Domae, Y. and Sakuratani, T. (2002). Alterations in photosynthesis and some antioxidant enzymatic activity of mungbean subjected to waterlogging. Journal of Plant Science. 163:117-123.
Armand, N., Amiri, H. and Ismaili, A. (2016). The effect of methanol on photosynthetic parameters of bean (Phaseolus vulgaris L.) under water deficit. Photosynthetica. 54: 288-294.
Ashraf, M. and Iram, A. (2005). Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Journal of Flora. 200: 535-546.
Bates, L.S., Waldern, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil Environment. 39: 205–207.
Benson, A.A. (1951). Identification of ribulose in 14CO2 photosynthetic products. Journal American Chemical Society. 73: 2971-2.
Bettaieb, I., Hamrouni-Sellami, I., Bourgou, S., Limam, F. and Marzouk, B. (2010). Drought effects on polyphenol composition and antioxidant activities in aerial parts of Salvia officinalis L. Acta Physiologiae Plantarum. 33(4):1103-1111.
Boscaiu, M., Sanchez, M., Bautista, I., Donat, P., Lidon, A., Llinares, J., Llul, C., Mayoral, O. and Vicente, O. (2010). Phenolic compounds as stress markers in plants from gypsum habitats. Bulletin of University of Agricultural Sciences and Veterinary. 67: 44-49.
Bradford, M.M. (1976). A rapid and sensitive method for quantitation of microgram of protein utilizing the principle of protein-dye binding. Analytical Biochemistry Quantities. 72: 248-254.
Chaves, M.M. (1991). Effects of water deficits on carbon assimilation. Journal of Experimental Botany. 42: 1-16.
Chaves, M.M. and Oliveira, M.M. (2004). Mechanisms underlying plant resilience to water deficits: Prospects for water-saving agriculture. Journal of Experimental Botany. 55: 2365-2384.
Downie, A., Miyazaki, S., Bohnert, H., John, P., Coleman, J., Parry, M. and Haslam, R. (2004). Expression profiling of the response of Arabidopsis thaliana to methanol stimulation. Phytochemistry. 65: 2305–2316.
Faver, K.L. and Gerik, T.J. (1996). Foliar-applied methanol effects on cotton(Gossypium hirsutum L.) gas exchange and growth. Field Crops Research. 47: 227–234.
Flexas, J. and Medrano, H. (2008). Drought-inhibition of photosynthesis in C3- plants: stomatal and nonstomatal limitation revisited. Annals of Botany. 183: 183-189.
Ghorbani, M. and Niakan, M. (2006). The effect of drought stress on soluble sugar, Total protein, proline, phenolic compound, chlorophyll content and rate reductase activity in Soybean (Glycine max L.cv.Gorgan3). Materials and Energy. 18(56):537-550
Gunes, A., Inal, A., Adak, M.S., Bagci, E.G., Cicek, N. and Eraslan, F. (2008). Effect of drought stress implemented at pre-or post-anthesis stage some physiological as screening criteria in chickpea cultivars. Russian Journal of Plant Physiology. 55: 59–67.
Haston, A.D. and Roje, S. (2001). One carbon metabolism in higher plants. Annual Reviw of Plant Biology. 52: 119-138.
Hare, P.D., Cress W.A. and Van Standen, J. (1998). Dissecting the rols of osmolyte accumulation during stress. Plant Cell Environment. 21: 535–553.
Hossinzadeh, S.R., Salimi, A., Ganjeali, A. and Ahmadpour, R. (2015). Effects of foliar application of methanol on biochemical characteristics and antioxidant enzyme activity of chickpea (Cicer arietinum L.) under drought stress. Plant Physiology and Biochemistry. 31(1):17-30.