تغییرات سیستم‌های پاداکساینده در انواع برگ‌های رنگی شمشاد طلایی (Euonymus japonicus Thunb.)

نوع مقاله: پژوهشی

نویسندگان

گروه زیست‌شناسی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

چکیده

برخی نشانگرهای فیزیولوژیکی و پاسخ­های آنتی­اکسیدان در انواع برگ­های رنگی درختچه ‍زینتی همیشه سبز شمشاد طلایی (Euonymus japonicus Thunb.) مورد مطالعه قرار گرفت. این درختچه دارای سه نوع برگ­ به رنگ­های سبز تیره، سبز روشن و زرد می­باشد. در این سه نوع برگ نشانگرهای تنش اکسیداتیو (غلظت پراکسید ­هیدروژن، پایداری غشاهای سلولی و پراکسیداسیون چربی­ها)، فعالیت آنزیم پراکسیداز و برخی ترکیبات آنتی­اکسیدان (ترکیبات فنلی، پرولین و اسیدهای آمینه آزاد) مطالعه شدند. نوع برگ بر محتوای پراکسید هیدروژن، میزان پایداری غشاهای سلولی و پراکسیداسیون چربی­های غشایی تاثیر معنی­دار گذاشت. مقدار اسیدهای­آمینه آزاد و پرولین در برگ­های زرد به شکل معنی­داری از برگ­های سبز تیره و روشن بیشتر بود. بیشترین و کمترین مقدار ترکیبات فنولی آزاد به ترتیب در برگ­های سبز تیره و زرد وجود داشت. یک الگوی افزایش فعالیت آنزیم پراکسیداز در برگ­های سبز روشن نسبت به سبز­تیره و برگ­های زرد نسبت به برگ­های سبز روشن مشاهده شد. از داده­های حاضر می­توان نتیجه گرفت که با وجود فعالیت سیستم­های آنتی­اکسیدان، تغییر رنگ برگ­ها از سبز تیره تا زرد منجر به افزایش آسیب­های اکسیداتیو می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Changes of antioxidative systems in various color leaves of Japanese spindle (Euonymus japonicus Thunb.)

نویسندگان [English]

  • Nader Chaparzadeh
  • Samane Safikhani
  • Laila Zarandi-Miandoab
Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran
چکیده [English]

Some physiological parameters and antioxidant responses were investigated in different color leaves of the evergreen shrub Japanese spindle (Euonymus japonica Thunb.). The shrub has leaves with three colors including dark green, light green, and yellow. Oxidative stress markers (hydrogen peroxide concentration, cell membrane stability, and lipids peroxidation), activity of peroxidase and some antioxidant molecules (phenolic compounds, proline, and free amino acids) were studied. Significant differences were found in hydrogen peroxide content, cell membrane stability, and membrane lipids peroxidation among leaves. In yellow leaves, the total content of proline and free amino acids were higher than those of the dark green and light green leaves. The highest and lowest free phenolic compounds contents were found in the dark green and yellow leaves, respectively. The POD activity increased significantly with changing leaf color from dark green to light green and to yellow. As a result, despite the activity of the antioxidant systems, the color change from dark green to yellow caused a gradual increase in the oxidative damage.

کلیدواژه‌ها [English]

  • Euonymus japonica
  • Hydrogen peroxide
  • Oxidative
  • phenolic compounds
  • Peroxidase

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