ارزیابی کارایی باکتری در تلقیح جداگانه و در ترکیب با باکتری حل کننده فسفات< Bacillus megaterium > بر کاهش تنش شوری در گیاه ماش

نوع مقاله: پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ارومیه، ایران

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه ایلام، ایران

چکیده

امروزه یکی از بزرگترین نگرانی‌های زراعت در مناطق خشک افزایش وسعت و میزان شوری اراضی کشاورزی است. در آزمایش مزرعه‌ای دوساله تاثیر کاربرد باکتری جنسAzospirillum در تلقیح به تنهایی و نیز در مخلوط با باکتریهای حل کننده فسفات بر کاهش تنش شوری بر گیاه ماش تحت شرایط مزرعه مورد بررسی قرار گرفت. طرح آزمایشی فاکتوریل در قالب بلوک‌های کامل تصادفی با سه تکرار بود. عامل‌های اصلی شامل تیمارهای کاربرد سویه‌های مختلف Azospirillu، باکتری‌های حل کننده فسفات (Bacillus megaterium) در تلقیح جداگانه و توامان با یکدیگر و نیز عدم تلقیح به عنوان شاهد و اعمال شوری آب آبیاری در دو سطح عدم اعمال شوری و شوری آب آبیاری به میزان 12 دسی‌زیمنس بر متر بود. نتایج نهایی تجزیه مرکب اثر اعمال شوری و نیز کاربرد کودهای زیستی نشان دهنده تاثیر معنی‌دار آنها بر عملکرد دانه ماش بود. شوری موجب کاهش 88 درصدی عملکرد نهایی دانه ماش گردید. تحت شوری و عدم شوری، بالاترین عملکرد دانه، تعداد دانه و وزن دانه را گیاهانی داشتند که بذر آنها با باکتری Azospirillu به تنهایی تلقیح گردیده بودند. کمترین محتوای سدیم و پتاسیم و بالاترین محتوای فسفر دانه را گیاهان تلقیح شده با باکتری Azospirillu داشتند. تلقیح دوگانه Azospirillu با Bacillus megaterium منجر به افزایش سودمندی باکتری Azospirillu در بهبود رشد گیاه ماش تحت هر دو شرایط شوری و غیر شوری نگردید. نتایج کلی این آزمایش نشان دهنده قابلیت به کارگیری Azospirillu در زراعت تحت شوری ماش بود هر چند تلفیق آن با باکتری Bacillus megaterium منجر به سودمندی بیشتری در این خصوص نگردید.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the effect of Azospirillum spp. inoculation, alone and in combination with phosphate solubilizing bacteria Bacillus megaterium , on alleviation of the effects of salinity stress on Mung bean (Vigna radiata L.)

نویسندگان [English]

  • Nosratollah Abasi 1
  • Jalal Jalilian 1
  • Mohhamad Javad Zare 2
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Urmia, Iran
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Ilam, Iran
چکیده [English]

Increasing salinity of soil and water is the most important concern for agriculture in dry lands. In this research the effect of Azospirillum inoculation alone and in co-inoculation with phosphate solubilizing bacteria was investigated in alleviating the adverse effect of salinity on mung bean Vigna radiata L. cv Gohar) for 2-year under field condition. The study was a factorial experiment based on a randomized complete block design with three replicates. The main factors were application of various serovars of Azospirillum, phosphate solubilizing bacteria Bacillus megaterium, co-inoculation of both bacteria, non-inoculated treatment as control and two levels of salinity irrigation (0.2 and 12 dS m-1). Analysis of variance indicated significant effect of salinity and microbial inoculation on mung bean seed yield. Salinity resulted in reduction of seed yield by 88%. Under salinity and non-salinity conditions, the highest amount of grain yield, seed weight, and seed number was observed in plants with seeds inoculated with Azospirillum. The minimum Na and K and maximum P contents of seeds were observed in Azospirillum inoculated plants. Co-inoculation with Azospirillum and Bacillus megaterium resulted in no further beneficial effect under saline and non-saline conditions. Overall, Azospirillum appeared to be effective in mitigation of the adverse effect of NaCl and its co-inoculation with phosphate solubilizing bacteria gained no more additives benefit.

کلیدواژه‌ها [English]

  • biofertilizer
  • Mung bean
  • Nutrient absorption
  • Salinity
  • Yield

 

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